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PeerJ. 2016 Sep 28;4:e2446. doi: 10.7717/peerj.2446. eCollection 2016.

XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves.

Author information

1
Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States.
2
Joint BioEnergy Institute, Emeryville, CA, United States.
3
Research School of Biology, Australian National University, Acton, Australia.
4
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Bejing, China.
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Contributed equally

Abstract

The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzaepv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis, we identified eight genes that are up-regulated in both in elf18 treated EFR:XA21:GFP rice leaves and Xoo infected XA21 rice leaves. These results provide a rapid and reliable method to assess bacterial-rice interactions.

KEYWORDS:

EFR; RNAseq; Rice; XA21; Xanthomonas

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