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PeerJ. 2016 Sep 28;4:e2446. doi: 10.7717/peerj.2446. eCollection 2016.

XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves.

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Department of Plant Pathology and the Genome Center, University of California, Davis, CA, United States.
Joint BioEnergy Institute, Emeryville, CA, United States.
Research School of Biology, Australian National University, Acton, Australia.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Bejing, China.
Contributed equally


The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzaepv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis, we identified eight genes that are up-regulated in both in elf18 treated EFR:XA21:GFP rice leaves and Xoo infected XA21 rice leaves. These results provide a rapid and reliable method to assess bacterial-rice interactions.


EFR; RNAseq; Rice; XA21; Xanthomonas

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