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Sci Rep. 2016 Oct 5;6:34800. doi: 10.1038/srep34800.

Optogenetic activation of septal GABAergic afferents entrains neuronal firing in the medial habenula.

Author information

1
Department of Biology, Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul, Republic of Korea.
2
Department of Molecular Biology, Sejong University, Seoul, Republic of Korea.

Abstract

The medial habenula (MHb) plays an important role in nicotine-related behaviors such as nicotine aversion and withdrawal. The MHb receives GABAergic input from the medial septum/diagonal band of Broca (MS/DB), yet the synaptic mechanism that regulates MHb activity is unclear. GABA (γ -aminobutyric acid) is a major inhibitory neurotransmitter activating both GABAA receptors and GABAB receptors. Depending on intracellular chloride concentration, however, GABAA receptors also function in an excitatory manner. In the absence of various synaptic inputs, we found that MHb neurons displayed spontaneous tonic firing at a rate of about ~4.4 Hz. Optogenetic stimulation of MS/DB inputs to the MHb evoked GABAA receptor-mediated synaptic currents, which produced stimulus-locked neuronal firing. Subsequent delayed yet lasting activation of GABAB receptors attenuated the intrinsic tonic firing. Consequently, septal GABAergic input alone orchestrates both excitatory GABAA and inhibitory GABAB receptors, thereby entraining the firing of MHb neurons.

PMID:
27703268
PMCID:
PMC5050514
DOI:
10.1038/srep34800
[Indexed for MEDLINE]
Free PMC Article

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