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Nat Rev Mol Cell Biol. 2017 Jan;18(1):5-17. doi: 10.1038/nrm.2016.121. Epub 2016 Oct 5.

Reverse-topology membrane scission by the ESCRT proteins.

Author information

1
Department of Molecular and Cell Biology and California Institute for Quantitative Biosciences, University of California, Berkeley, California 94720, USA.
2
University of Utah, Salt Lake City, Utah 84112, USA.
3
Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.

Abstract

The narrow membrane necks formed during viral, exosomal and intra-endosomal budding from membranes, as well as during cytokinesis and related processes, have interiors that are contiguous with the cytosol. Severing these necks involves action from the opposite face of the membrane as occurs during the well-characterized formation of coated vesicles. This 'reverse' (or 'inverse')-topology membrane scission is carried out by the endosomal sorting complex required for transport (ESCRT) proteins, which form filaments, flat spirals, tubes and conical funnels that are thought to direct membrane remodelling and scission. Their assembly, and their disassembly by the ATPase vacuolar protein sorting-associated 4 (VPS4) have been intensively studied, but the mechanism of scission has been elusive. New insights from cryo-electron microscopy and various types of spectroscopy may finally be close to rectifying this situation.

PMID:
27703243
PMCID:
PMC5198518
DOI:
10.1038/nrm.2016.121
[Indexed for MEDLINE]
Free PMC Article

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