Format

Send to

Choose Destination
Blood. 2016 Dec 29;128(26):3125-3136. doi: 10.1182/blood-2016-02-697003. Epub 2016 Oct 4.

miR-125b controls monocyte adaptation to inflammation through mitochondrial metabolism and dynamics.

Author information

1
INSERM, U1183, CHU Saint Eloi, Montpellier, France.
2
University of Medicine, Montpellier, France.
3
Exploratory Unit, Sanofi R&D, Montpellier, France.
4
Deutsches Rheuma-Forschungszentrum Berlin, an Institute of the Leibniz Association, Berlin, Germany.
5
Rheumatologie Department, Charité Universitätsmedizin Berlin, Berlin, Germany.
6
Institut de génétique moléculaire de Montpellier, CNRS Unité mixte de recherche 5535, Unité mixte de service France, Montpellier, France.
7
Institut Necker-Enfants Malades, INSERM, U1151, CNRS Unité mixte de recherche 8253, Université Paris Descartes-Sorbonne Paris Cité, Paris, France.
8
Unité de Sciences Translationnelles, Sanofi, Chilly-Mazarin, France; and.
9
Clinical Department for Osteoarticular Diseases, CHU Lapeyronie, Montpellier, France.

Abstract

Metabolic changes drive monocyte differentiation and fate. Although abnormal mitochondria metabolism and innate immune responses participate in the pathogenesis of many inflammatory disorders, molecular events regulating mitochondrial activity to control life and death in monocytes remain poorly understood. We show here that, in human monocytes, microRNA-125b (miR-125b) attenuates the mitochondrial respiration through the silencing of the BH3-only proapoptotic protein BIK and promotes the elongation of the mitochondrial network through the targeting of the mitochondrial fission process 1 protein MTP18, leading to apoptosis. Proinflammatory activation of monocyte-derived macrophages is associated with a concomitant increase in miR-125b expression and decrease in BIK and MTP18 expression, which lead to reduced oxidative phosphorylation and enhanced mitochondrial fusion. In a chronic inflammatory systemic disorder, CD14+ blood monocytes display reduced miR-125b expression as compared with healthy controls, inversely correlated with BIK and MTP18 messenger RNA expression. Our findings not only identify BIK and MTP18 as novel targets for miR-125b that control mitochondrial metabolism and dynamics, respectively, but also reveal a novel function for miR-125b in regulating metabolic adaptation of monocytes to inflammation. Together, these data unravel new molecular mechanisms for a proapoptotic role of miR-125b in monocytes and identify potential targets for interfering with excessive inflammatory activation of monocytes in inflammatory disorders.

PMID:
27702798
PMCID:
PMC5335801
DOI:
10.1182/blood-2016-02-697003
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center