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Toxicol Sci. 2017 Jan;155(1):248-257. doi: 10.1093/toxsci/kfw198. Epub 2016 Oct 3.

Molecular Impact of Electronic Cigarette Aerosol Exposure in Human Bronchial Epithelium.

Author information

1
Section of Computational Biomedicine, Department of Medicine Boston University School of Medicine, Boston, Massachusetts 02118.
2
MatTek Corporation, Ashland, Massachusetts 01721.
3
University of California Los Angeles, Los Angeles, California 90095.
4
Department of Epidemiology, Boston University School of Public Health, Boston, Massachusetts 02118.
5
Pulmonary Center, Boston University School of Medicine, Boston, Massachusetts 02118.
6
Section of Computational Biomedicine, Department of Medicine Boston University School of Medicine, Boston, Massachusetts 02118; mlenburg@bu.edu.

Abstract

Little evidence is available regarding the physiological effects of exposure to electronic cigarette (ECIG) aerosol. We sought to determine the molecular impact of ECIG aerosol exposure in human bronchial epithelial cells (HBECs). Gene-expression profiling was conducted in primary grown at air liquid interface and exposed to 1 of 4 different ECIG aerosols, traditional tobacco cigarette (TCIG) smoke, or clean air. Findings were validated experimentally with quantitative polymerase chain reaction and a reactive oxygen species immunoassay. Using gene set enrichment analysis, signatures of in vitro ECIG exposure were compared with those generated from bronchial epithelial brushings of current TCIG smokers and former TCIG smokers currently using ECIGs. We found 546 genes differentially expressed across the ECIG, TCIG, and air-exposed groups of HBECs (ANOVA; FDR q < .05; fold change > 1.5). A subset of these changes were shared between TCIG- and ECIG-exposed HBECs. ECIG exposure induced genes involved in oxidative and xenobiotic stress pathways and increased a marker of reactive oxygen species production in a dose-dependent manner. ECIG exposure decreased expression of genes involved in cilia assembly and movement. Furthermore, gene-expression differences observed in vitro were concordant with differences observed in airway epithelium collected from ECIG users (q < .01). In summary, our data suggest that ECIG aerosol can induce gene-expression changes in bronchial airway epithelium in vitro, some of which are shared with TCIG smoke. These changes were generally less pronounced than the effects of TCIG exposure and were more pronounced in ECIG products containing nicotine than those without nicotine. Our data further suggest that the gene-expression alterations seen with the in vitro exposure system reflects the physiological effects experienced in vivo by ECIG users.

KEYWORDS:

electronic cigarette.; gene expression; smoke; tobacco

PMID:
27701119
PMCID:
PMC5216655
DOI:
10.1093/toxsci/kfw198
[Indexed for MEDLINE]
Free PMC Article

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