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Biomed Opt Express. 2016 Aug 29;7(9):3716-3720. eCollection 2016 Sep 1.

3D imaging in CUBIC-cleared mouse heart tissue: going deeper.

Author information

1
Instituto de Investigación Sanitaria Gregorio Marañón. (IiSGM), Madrid, Spain; These authors contributed equally to this work.
2
Instituto de Investigación Sanitaria Gregorio Marañón. (IiSGM), Madrid, Spain; Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, Spain; These authors contributed equally to this work.
3
Instituto de Investigación Sanitaria Gregorio Marañón. (IiSGM), Madrid, Spain; CIBER de Enfermedades Hepáticas y Digestivas (CIBERehd), Madrid, Spain.
4
Instituto de Investigación Sanitaria Gregorio Marañón. (IiSGM), Madrid, Spain; Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, Spain.
5
Instituto de Investigación Sanitaria Gregorio Marañón. (IiSGM), Madrid, Spain; Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, Spain; Centro de Investigación Biomédica en Red de Salud Mental (CIBERSAM), Madrid, Spain.

Abstract

The ability to acquire high resolution 3D images of the heart enables to study heart diseases more in detail. In this work, the CUBIC (clear, unobstructed brain imaging cocktails and computational analysis) clearing protocol was optimized for thick mouse heart sections to enhance the penetration depth of the confocal microscope lasers into the tissue. In addition, the optimized CUBIC clearing of the heart enhances antibody penetration into the tissue by a factor of five. The present protocol enables deep 3D high-quality image acquisition in the heart allowing a much more accurate assessment of the cellular and structural changes that underlie heart diseases.

KEYWORDS:

(000.1430) Biology and medicine; (180.1790) Confocal microscopy

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