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Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):E5783-E5791.

Negative cooperativity in the nitrogenase Fe protein electron delivery cycle.

Author information

1
Department of Chemistry and Biochemistry, Utah State University, Logan, UT 84322.
2
Department of Chemistry, Northwestern University, Evanston, IL 60208.
3
Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061.
4
Catalysis Science, Physical Science Division, Pacific Northwestern National Laboratory, Richland, WA 99352.
5
Department of Chemistry, Northwestern University, Evanston, IL 60208; bmh@northwestern.edu lance.seefeldt@usu.edu edwin.antony@marquette.edu.
6
Department of Chemistry and Biochemistry, Utah State University, Logan, UT 84322; bmh@northwestern.edu lance.seefeldt@usu.edu edwin.antony@marquette.edu.
7
Department of Biological Sciences, Marquette University, Milwaukee, WI 53201 bmh@northwestern.edu lance.seefeldt@usu.edu edwin.antony@marquette.edu.

Abstract

Nitrogenase catalyzes the ATP-dependent reduction of dinitrogen (N2) to two ammonia (NH3) molecules through the participation of its two protein components, the MoFe and Fe proteins. Electron transfer (ET) from the Fe protein to the catalytic MoFe protein involves a series of synchronized events requiring the transient association of one Fe protein with each αβ half of the α2β2 MoFe protein. This process is referred to as the Fe protein cycle and includes binding of two ATP to an Fe protein, association of an Fe protein with the MoFe protein, ET from the Fe protein to the MoFe protein, hydrolysis of the two ATP to two ADP and two Pi for each ET, Pi release, and dissociation of oxidized Fe protein-(ADP)2 from the MoFe protein. Because the MoFe protein tetramer has two separate αβ active units, it participates in two distinct Fe protein cycles. Quantitative kinetic measurements of ET, ATP hydrolysis, and Pi release during the presteady-state phase of electron delivery demonstrate that the two halves of the ternary complex between the MoFe protein and two reduced Fe protein-(ATP)2 do not undergo the Fe protein cycle independently. Instead, the data are globally fit with a two-branch negative-cooperativity kinetic model in which ET in one-half of the complex partially suppresses this process in the other. A possible mechanism for communication between the two halves of the nitrogenase complex is suggested by normal-mode calculations showing correlated and anticorrelated motions between the two halves.

KEYWORDS:

ATP hydrolysis; allosteric control; conformational control; half-sites reactivity

PMID:
27698129
PMCID:
PMC5056064
DOI:
10.1073/pnas.1613089113
[Indexed for MEDLINE]
Free PMC Article

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