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Int Immunopharmacol. 2016 Nov;40:428-435. doi: 10.1016/j.intimp.2016.09.022. Epub 2016 Sep 30.

Prevention of azoxymethane/dextran sodium sulfate-induced mouse colon carcinogenesis by processed Aloe vera gel.

Author information

1
College of Pharmacy, Chungbuk National University, Cheongju 28644, South Korea.
2
Univera Inc., Seoul 04782, South Korea.
3
College of Pharmacy, Korea University, Sejong 30019, South Korea.
4
College of Pharmacy, Chungbuk National University, Cheongju 28644, South Korea. Electronic address: cklee@chungbuk.ac.kr.

Abstract

The preventive effect of a processed Aloe vera gel (PAG) on colon carcinogenesis was examined using an azoxymethane (AOM)-initiated and dextran sodium sulfate (DSS)-promoted mouse colon carcinogenesis model. Oral administration of PAG (200, or 400mg/kg/day) significantly reduced the multiplicity of colonic adenomas and adenocarcinomas compared with the AOM/DSS only-treated mice. In the mice treated with 400mg/kg of PAG, adenoma and adenocarcinoma development was reduced to 80% and 60%, respectively, compared to 100% in the PAG-untreated AOM/DSS-treated mice. Western blot analysis using colon extracts showed that PAG reduced the activation of nuclear factor kappa B (NF-κB), resulting in the inhibition of inducible nitric oxide synthase and cyclooxygenase-2 expression. PAG appeared to inhibit the NF-κB activation through the activation of peroxisome proliferator-activated receptor gamma. PAG also inhibited the expression and phosphorylation of signal transducer and activator of transcription 3, which is known to connect inflammation and cancer. In addition, PAG inhibited cell cycle progression-inducing cellular factors, such as extracellular signal-regulated kinases 1/2, cyclin-dependent kinase 4, and cyclin D1. On the other hand, PAG increased the expression of Caudal-related homeobox transcription factor 2, which is known to be a tumor suppressor in colorectal cancer. These findings show that PAG suppresses colitis-related colon carcinogenesis by inhibiting both chronic inflammation and cell cycle progression in the colon.

KEYWORDS:

Cell cycle progression; Chronic inflammation; Colon carcinogenesis; Myeloid-derived suppressor cells; Processed Aloe vera gel

PMID:
27697726
DOI:
10.1016/j.intimp.2016.09.022
[Indexed for MEDLINE]

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