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Head Neck. 2017 Feb;39(2):341-346. doi: 10.1002/hed.24593. Epub 2016 Oct 3.

Copy number profiling of tumor suppressor genes in head and neck cancer.

Author information

1
Department of Basic Oncology, Oncology Institute, Istanbul University, Istanbul, Turkey.
2
Department of Otorhinolaryngology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey.

Abstract

BACKGROUND:

Sensitive and reliable new biomarkers are needed in head and neck cancer to predict the outcome and for therapy that is more effective. Copy number alterations are frequent and play a critical role in cancer.

METHODS:

Copy number alterations of 24 tumor suppressor genes in head and neck cancer were analyzed simultaneously in matched tumor and normal samples from 93 patients using multiplex ligation-dependent probe amplification (MLPA).

RESULTS:

Chromosomes 3p and 9p displayed the most common alterations. The gene displaying most frequent losses was the mutL homolog 1 (MLH1) gene, followed by the cyclin-dependent kinase inhibitor 2A (CDKN2A) and CDKN2B genes. A significant correlation was observed between the CDKN2A and CDKN2B genes. The tissue inhibitor of metalloproteinase (TIMP)3 gene alterations were observed in 8 tumors.

CONCLUSION:

Our data confirm previous observations and suggest that losses of the MLH1 and CDKN2 genes and alterations of the TIMP3 gene play an important role in head and neck carcinogenesis. © 2016 Wiley Periodicals, Inc. Head Neck 39: 341-346, 2017.

KEYWORDS:

copy number change; head and neck cancer; multiplex ligation-dependent probe amplification (MLPA) analysis; tissue inhibitor of metalloproteinase 3 (TIMP3) amplification; tumor suppressor genes

PMID:
27696595
DOI:
10.1002/hed.24593
[Indexed for MEDLINE]

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