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Photodermatol Photoimmunol Photomed. 2017 Jan;33(1):49-57. doi: 10.1111/phpp.12276. Epub 2016 Nov 3.

Inhibitory effect of 660-nm LED on melanin synthesis in in vitro and in vivo.

Author information

1
Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Korea.
2
Department of Medicine, Graduate School, Chung-Ang University, Seoul, Korea.
3
Department of Otorhinolaryngology, Chung-Ang University College of Medicine, Seoul, Korea.
4
Department of Dermatology, College of Medicine, Catholic Kwandong University, International St. Mary's Hospital, Incheon, Korea.
5
Medical IT Convergence Research Center, Korea Electronics Technology Institute, Gyeonggi-do, Korea.

Abstract

BACKGROUND:

Skin hyperpigmentary disorders including postinflammatory hyperpigmentation, melasma, solar lentigines, and conditions like freckles are common. The light-emitting diodes (LEDs) are the latest category of nonthermal and noninvasive phototherapy to be considered in skin pigmentation disorder treatment.

PURPOSE:

The purpose of this study was to investigate the effects of 660-nm LED on inhibition of melanogenesis. We investigated whether a 660-nm LED affected melanin synthesis in in vitro and in vivo models, and we explored the mechanisms involved.

METHODS:

The inhibitory effect of 660-nm LED on melanin synthesis was evaluated in B16F10 cells and HRM-2 melanin-possessing hairless mice were used to evaluate the antimelanogenic effects of 660-nm LED.

RESULTS:

Interestingly, 660-nm LED inhibited alpha-melanocyte-stimulating hormone-induced tyrosinase activity in B16F10 cells. We also found that 660-nm LED decreased MITF and tyrosinase expression and induced the activation of ERK. These findings suggest that the depigmenting effects of 660-nm LED result from downregulation of MITF and tyrosinase expression due to increased ERK activity. The 660-nm LED reduced UVB-induced melanogenesis in the skin of HRM-2 via downregulation of tyrosinase and MITF.

CONCLUSION:

These findings suggest 660-nm LED is a potentially depigmentation strategy.

KEYWORDS:

660-nm wavelength; B16F10 cell; HRM-2; light-emitting diode; melanogenesis

PMID:
27696509
DOI:
10.1111/phpp.12276
[Indexed for MEDLINE]

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