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Nat Commun. 2016 Oct 3;7:12896. doi: 10.1038/ncomms12896.

A plasma membrane microdomain compartmentalizes ephrin-generated cAMP signals to prune developing retinal axon arbors.

Averaimo S1,2,3, Assali A4,5,6, Ros O1,2,3, Couvet S1,2,3, Zagar Y1,2,3, Genescu I1,2,3, Rebsam A4,5,6, Nicol X1,2,3.

Author information

Sorbonne Universités, UPMC University Paris 06, UMR_S 968, Institut de la Vision, Paris F-75012, France.
CNRS, UMR_7210, Paris F-75012, France.
INSERM, UMR_S 968, Paris F-75012, France.
Sorbonne Universités, UPMC University Paris 06, UMR_S 839, Paris F-75005, France.
INSERM UMR_S 839, Paris F-75005, France.
Institut du Fer à Moulin, Paris F-75005, France.


The development of neuronal circuits is controlled by guidance molecules that are hypothesized to interact with the cholesterol-enriched domains of the plasma membrane termed lipid rafts. Whether such domains enable local intracellular signalling at the submicrometre scale in developing neurons and are required for shaping the nervous system connectivity in vivo remains controversial. Here, we report a role for lipid rafts in generating domains of local cAMP signalling in axonal growth cones downstream of ephrin-A repulsive guidance cues. Ephrin-A-dependent retraction of retinal ganglion cell axons involves cAMP signalling restricted to the vicinity of lipid rafts and is independent of cAMP modulation outside of this microdomain. cAMP modulation near lipid rafts controls the pruning of ectopic axonal branches of retinal ganglion cells in vivo, a process requiring intact ephrin-A signalling. Together, our findings indicate that lipid rafts structure the subcellular organization of intracellular cAMP signalling shaping axonal arbors during the nervous system development.

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