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J Evid Based Complementary Altern Med. 2017 Jul;22(3):407-412. doi: 10.1177/2156587216668110. Epub 2016 Sep 30.

Tribulus terrestris Extract Improves Human Sperm Parameters In Vitro.

Author information

1
1 Medical Biology Research Center, Kermanshah University of medical sciences, Kermanshah, Iran.
2
2 Fertility & Infertility Research Center, Kermanshah University of Medical Sciences, Sorkheh Lizheh, Kermanshah, Iran.

Abstract

OBJECTIVE:

The object of present study was to investigate the effects of direct addition of Tribulus terrestris extract on human sperm parameters.

DESIGN:

Semen specimens from 40 healthy men volunteers were divided into 4 groups: one group received no treatment (control group) while the others were incubated with 20, 40, and 50 µg/mL of T terrestris extract (experimental groups). Motility, viability, and DNA fragmentation were assessed in all groups.

RESULTS:

The incubation of human semen with 40 and 50 μg/mL of T terrestris extract significantly enhanced total sperm motility, number of progressive motile spermatozoa, and curvilinear velocity over 60 to 120 minutes' holding time ( P < .05 or P < < .01). Furthermore, viability was significantly enhanced by using T terrestris extract ( P < .01).

CONCLUSIONS:

In vitro addition of the T terrestris extract to human sperm could affect male fertility capacity.

KEYWORDS:

DNA fragmentation; Tribulus terrestris; human sperm; motility; viability

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