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Plant Physiol. 2016 Nov;172(3):1596-1611. Epub 2016 Sep 30.

A Tomato Vacuolar Invertase Inhibitor Mediates Sucrose Metabolism and Influences Fruit Ripening.

Qin G1,2,3, Zhu Z1,2,3, Wang W1,2,3, Cai J1,2,3, Chen Y1,2,3, Li L1,2,3, Tian S4,5,6.

Author information

1
Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Xiangshan, Haidian District, Beijing 100093, China (G.Q., Z.Z., W.W., J.C., Y.C., S.T.).
2
University of Chinese Academy of Sciences, Yuquanlu, Beijing 100049, China (W.W., J.C., Y.C., S.T.); and.
3
Robert W. Holley Center for Agriculture and Health, Agricultural Research Service, United States Department of Agriculture, Ithaca, New York 14853 (L.L.).
4
Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Xiangshan, Haidian District, Beijing 100093, China (G.Q., Z.Z., W.W., J.C., Y.C., S.T.); tsp@ibcas.ac.cn.
5
University of Chinese Academy of Sciences, Yuquanlu, Beijing 100049, China (W.W., J.C., Y.C., S.T.); and tsp@ibcas.ac.cn.
6
Robert W. Holley Center for Agriculture and Health, Agricultural Research Service, United States Department of Agriculture, Ithaca, New York 14853 (L.L.) tsp@ibcas.ac.cn.

Abstract

Fruit ripening is a complex process that involves a series of physiological and biochemical changes that ultimately influence fruit quality traits, such as color and flavor. Sugar metabolism is an important factor in ripening, and there is evidence that it influences various aspects of ripening, although the associated mechanism is not well understood. In this study, we identified and analyzed the expression of 36 genes involved in Suc metabolism in ripening tomato (Solanum lycopersicum) fruit. Chromatin immunoprecipitation and gel mobility shift assays indicated that SlVIF, which encodes a vacuolar invertase inhibitor, and SlVI, encoding a vacuolar invertase, are directly regulated by the global fruit ripening regulator RIPENING INHIBITOR (RIN). Moreover, we showed that SlVIF physically interacts with SlVI to control Suc metabolism. Repression of SlVIF by RNA interference delayed tomato fruit ripening, while overexpression of SlVIF accelerated ripening, with concomitant changes in lycopene production and ethylene biosynthesis. An isobaric tags for relative and absolute quantification-based quantitative proteomic analysis further indicated that the abundance of a set of proteins involved in fruit ripening was altered by suppressing SlVIF expression, including proteins associated with lycopene generation and ethylene synthesis. These findings provide evidence for the role of Suc in promoting fruit ripening and establish that SlVIF contributes to fruit quality and the RIN-mediated ripening regulatory mechanisms, which are of significant agricultural value.

PMID:
27694342
PMCID:
PMC5100769
DOI:
10.1104/pp.16.01269
[Indexed for MEDLINE]
Free PMC Article

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