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Biomed Pharmacother. 2016 Dec;84:583-591. doi: 10.1016/j.biopha.2016.09.056. Epub 2016 Sep 29.

Down-regulation of microRNA-338-3p promoted angiogenesis in hepatocellular carcinoma.

Author information

1
Department of Hepatic Surgery and Liver transplantation Center of the Third Affiliated Hospital, Organ Transplantation Institute of Sun Yat-sen University, Organ Transplantation Research Center of Guangdong Province, Guangzhou 510630, China. Electronic address: zhjeff72@sina.com.
2
Guangdong Provincial Key Laboratory of Liver Disease Research, Guangzhou, Guangdong 510630, China ; Key Laboratory of Clinical & Translational Research on Biotherapy for Liver Disease of Guangdong Higher Education Institutes, Sun Yat-sen University ; Cell-Gene Therapy Translational Medicine Research Center of the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510630, China. Electronic address: uv1@163.com.
3
Department of General Surgery, the Second People's Hospital of Guangdong Province, Guangzhou 510317, China. Electronic address: zxcq12333@163.com.
4
Department of Hepatic Surgery and Liver transplantation Center of the Third Affiliated Hospital, Organ Transplantation Institute of Sun Yat-sen University, Organ Transplantation Research Center of Guangdong Province, Guangzhou 510630, China. Electronic address: njiang163@163.com.
5
Department of Hepatic Surgery and Liver transplantation Center of the Third Affiliated Hospital, Organ Transplantation Institute of Sun Yat-sen University, Organ Transplantation Research Center of Guangdong Province, Guangzhou 510630, China. Electronic address: andyfu125@163.com.
6
Department of Hepatic Surgery and Liver transplantation Center of the Third Affiliated Hospital, Organ Transplantation Institute of Sun Yat-sen University, Organ Transplantation Research Center of Guangdong Province, Guangzhou 510630, China. Electronic address: gy3099@126.com.
7
Department of Hepatic Surgery and Liver transplantation Center of the Third Affiliated Hospital, Organ Transplantation Institute of Sun Yat-sen University, Organ Transplantation Research Center of Guangdong Province, Guangzhou 510630, China. Electronic address: ylhmin@hotmail.com.
8
Department of Hepatic Surgery and Liver transplantation Center of the Third Affiliated Hospital, Organ Transplantation Institute of Sun Yat-sen University, Organ Transplantation Research Center of Guangdong Province, Guangzhou 510630, China. Electronic address: lihua100@yeah.net.
9
Guangdong Provincial Key Laboratory of Liver Disease Research, Guangzhou, Guangdong 510630, China ; Key Laboratory of Clinical & Translational Research on Biotherapy for Liver Disease of Guangdong Higher Education Institutes, Sun Yat-sen University ; Cell-Gene Therapy Translational Medicine Research Center of the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510630, China. Electronic address: kee_kee@126.com.
10
Guangdong Provincial Key Laboratory of Liver Disease Research, Guangzhou, Guangdong 510630, China ; Key Laboratory of Clinical & Translational Research on Biotherapy for Liver Disease of Guangdong Higher Education Institutes, Sun Yat-sen University ; Cell-Gene Therapy Translational Medicine Research Center of the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510630, China. Electronic address: chenwenjie168@soho.com.
11
Department of Hepatic Surgery and Liver transplantation Center of the Third Affiliated Hospital, Organ Transplantation Institute of Sun Yat-sen University, Organ Transplantation Research Center of Guangdong Province, Guangzhou 510630, China. Electronic address: chgh1955@263.net.

Abstract

miRNAs are involved in substantial biological passways, including tumorigenesis, cancer development and progression. Angiogenesis plays a vital role in the progression of hepatocellular carcinoma (HCC), and VEGF is closely associated with the angiogenesis. However, the molecular mechanism of miRNAs in regulation tumorigenesis of HCC remains to be investigated. In the present research, we confirmed that miR-338-3p was suppressed both in HCC tissues and HCC cell lines. Then the tube formation, transwell and Chorioallantoic membrane (CAM) assay were carried out, such indicated that down-regulation of miR-338-3p can sharply increased, while up-regulation drastically suppressed angiogenesis of HCC cells in vitro. Moreover, MACC1 is predicted to be a target of miR-338-3p and we checked the prediction through luciferase assay. And then, our research showed that negative correlation existed between miR-338-3p and MACC1, β-catenin and VEGF that has been reported participated in cancer behavior in HCC cell lines. Subsequently, our assays illustrated that suppression miR-338-3p can up-regulate MACC1, β-catenin and VEGF expression of HCC cells. In conclusion, our research discovered that miR-338-3p can contribute to HCC angiogenesis by targeting MACC1, β-catenin and VEGF.

KEYWORDS:

Angiogenesis; Hepatocellular carcinoma; VEGF; microRNA

PMID:
27694002
DOI:
10.1016/j.biopha.2016.09.056
[Indexed for MEDLINE]

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