Format

Send to

Choose Destination
Biomed Pharmacother. 2016 Dec;84:559-568. doi: 10.1016/j.biopha.2016.09.069. Epub 2016 Sep 29.

Neuroprotective effects of quercetin on memory and anxiogenic-like behavior in diabetic rats: Role of ectonucleotidases and acetylcholinesterase activities.

Author information

1
Programa de Pós-Graduação em Medicina Veterinária, Laboratório de Análises Clínicas Veterinária, Centro de Ciências Rurais, Universidade Federal de Santa Maria, Santa Maria/RS 97105-900, Brazil.
2
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria/RS 97105-900, Brazil. Electronic address: fabiseco@yahoo.com.br.
3
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria/RS 97105-900, Brazil; Department of Medical Biochemistry, Afe Babalola University, Ado Ekiti, P.M.B 5454. Ado Ekiti, Nigeria.
4
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria/RS 97105-900, Brazil.
5
Programa de Pós-Graduação em Medicina Veterinária, Laboratório de Análises Clínicas Veterinária, Centro de Ciências Rurais, Universidade Federal de Santa Maria, Santa Maria/RS 97105-900, Brazil; Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria/RS 97105-900, Brazil.
6
Programa de Pós-Graduação em Ciências Odontológicas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
7
Programa de Pós-Graduação em Medicina Veterinária, Laboratório de Análises Clínicas Veterinária, Centro de Ciências Rurais, Universidade Federal de Santa Maria, Santa Maria/RS 97105-900, Brazil. Electronic address: sonia@smail.ufsm.br.

Abstract

The present study investigated the protective effect of quercetin (Querc) on memory, anxiety-like behavior and impairment of ectonucleotidases and acetylcholinesterase (AChE) activities in brain of streptozotocin-induced diabetic rats (STZ-diabetes). The type 1 diabetes mellitus was induced by an intraperitoneal injection of 70mg/kg of streptozotocin (STZ), diluted in 0.1M sodium-citrate buffer (pH 4.5). Querc was dissolved in 25% ethanol and administered by gavage at the doses of 5, 25 and 50mg/kg once a day during 40days. The animals were distributed in eight groups of ten animals as follows: vehicle, Querc 5mg/kg, Querc 25mg/kg, Querc 50mg/kg, diabetes, diabetes plus Querc 5mg/kg, diabetes plus Querc 25mg/kg and diabetes plus Querc 50mg/kg. Querc was able to prevent the impairment of memory and the anxiogenic-like behavior induced by STZ-diabetes. In addition, Querc prevents the decrease in the NTPDase and increase in the adenosine deaminase (ADA) activities in SN from cerebral cortex of STZ-diabetes. STZ-diabetes increased the AChE activity in SN from cerebral cortex and hippocampus. Querc 50mg/kg was more effective to prevent the increase in AChE activity in the brain of STZ-diabetes. Querc also prevented an increase in the malondialdehyde levels in all the brain structures. In conclusion, the present findings showed that Querc could prevent the impairment of the enzymes that regulate the purinergic and cholinergic extracellular signaling and improve the memory and anxiety-like behavior induced by STZ-diabetes.

KEYWORDS:

Acetylcholinesterase; Anxiety; Diabetes; Ectonucleotidases; Memory; Quercetin

PMID:
27694000
DOI:
10.1016/j.biopha.2016.09.069
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center