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Virus Res. 2017 Jan 2;227:110-114. doi: 10.1016/j.virusres.2016.09.020. Epub 2016 Sep 29.

Identification of heat shock protein A9 as a Tembusu virus binding protein on DF-1 cells.

Author information

1
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, Jiangsu, China.
2
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, Jiangsu, China. Electronic address: muziyin15@163.com.

Abstract

This study attempts to identify receptor elements for Tembusu virus (TMUV) on DF-1 cells. Using co-immunoprecipitation and virus overlay protein binding assays, we identified a TMUV-binding protein of approximately 70-kDa on DF-1 cell membranes. Mass spectroscopy identified the protein to be heat shock protein (HSP) A9, which was reconfirmed by an anti-HSPA9 antibody. Indirect immunofluorescence demonstrated a significant degree of colocalization between HSPA9 and TMUV on cell surface. Additionally, an antibody against HSPA9 could inhibit TMUV infection in DF-1 cells in a dose-dependent manner. These results might suggest that HSPA9 is a putative receptor for TMUV.

KEYWORDS:

Heat shock protein A9; Receptor; Tembusu virus

PMID:
27693918
DOI:
10.1016/j.virusres.2016.09.020
[Indexed for MEDLINE]

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