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Int J Biol Macromol. 2017 Jan;94(Pt A):1-9. doi: 10.1016/j.ijbiomac.2016.09.099. Epub 2016 Sep 29.

Effects of polysaccharide from mycelia of Ganoderma lucidum on intestinal barrier functions of rats.

Author information

1
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, PR China. Electronic address: mljin@nwpu.edu.cn.
2
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, PR China.
3
College of Medicine, Xi'an Jiaotong University, No. 76 Yanta West Road, Xi'an, 710061, PR China.

Abstract

The intestinal mucosal barriers play essential roles not only in the digestion and absorption of nutrients, but also the innate defense against most intestinal pathogens. In the present study, polysaccharide from the mycelia of Ganoderma lucidum was given via oral administration to rats (100mg/kg body weight, 21days) to investigate its effects on intestinal barrier functions, including the mechanical barrier, immunological barrier and biological barrier function. It was found that the polysaccharide administration could significantly up-regulate the expression of occludin, nuclear factor-κB p65 (NF-κB p65) and secretory immunoglobulin A (SIgA) in ileum, markedly improve the levels of interferon-γ (IFN-γ), interleukin-2 (IL-2), and IL-4, and decrease the level of diamine oxidase (DAO) in serum. Meanwhile, rats from the polysaccharide group showed significant higher microbiota richness in cecum as reflected by the Chao 1 index compared with the control group. Moreover, the polysaccharide decreased the Firmicutes-to-Bacteroidetes ratio. Our results indicated that the polysaccharide from the mycelia of G. lucidum might be used as functional agent to regulate the intestinal barrier functions.

KEYWORDS:

Ganoderma lucidum; Intestinal barrier function; Polysaccharide

PMID:
27693834
DOI:
10.1016/j.ijbiomac.2016.09.099
[Indexed for MEDLINE]

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