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Life Sci. 2016 Nov 15;165:100-108. doi: 10.1016/j.lfs.2016.09.023. Epub 2016 Sep 28.

Effects of physical exercise on myokines expression and brown adipose-like phenotype modulation in rats fed a high-fat diet.

Author information

1
CIAFEL - Research Centre in Physical Activity, Health and Leisure, Faculty of Sport, University of Porto, Porto, Portugal. Electronic address: silviadarocharodrigues@gmail.com.
2
Metabolic Research Laboratory, Clínica Universidad de Navarra, Pamplona, Spain; Obesity & Adipobiology Group, Instituto de Investigación Sanitario de Navarra (IdiSNA), Pamplona, Spain; CIBEROBN, Instituto de Salud Carlos III, Pamplona, Spain.
3
Department of Experimental Biology, Faculty of Medicine, University of Porto, Porto, Portugal; Instituto de Investigação e Inovação em Saúde, Institute for Molecular and Cell Biology (IBMC), University of Porto, Porto, Portugal.
4
CIAFEL - Research Centre in Physical Activity, Health and Leisure, Faculty of Sport, University of Porto, Porto, Portugal.
5
Metabolic Research Laboratory, Clínica Universidad de Navarra, Pamplona, Spain; Department of Endocrinology & Nutrition, Clínica Universidad de Navarra, Pamplona, Spain; Obesity & Adipobiology Group, Instituto de Investigación Sanitario de Navarra (IdiSNA), Pamplona, Spain; CIBEROBN, Instituto de Salud Carlos III, Pamplona, Spain.

Abstract

AIMS:

Exercise-stimulated myokine secretion into circulation may be related with browning in white adipose tissue (WAT), representing a positive metabolic effect on whole-body fat mass. However, limited information is yet available regarding the impact of exercise on myokine-related modulation of adipocyte phenotype in WAT from obese rats.

MAIN METHODS:

Sprague-Dawley rats (n=60) were divided into sedentary and voluntary physical activity (VPA) groups and fed with standard (35kcal% fat) or high-fat (HFD, 71kcal% fat)-isoenergetic diets. The VPA-groups had unrestricted access to wheel running throughout the protocol. After-9weeks, half of sedentary standard (SS) and sedentary HFD (HS)-fed animals were exercised on treadmill (endurance training, ET) for 8-weeks while maintaining the dietary treatments.

KEY FINDINGS:

The adipocyte hypertrophy induced by HFD were attenuated by VPA and ET. HFD decreased 5' AMP-activated protein kinase (AMPK) activity in muscle as well as peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) and uncoupling protein 1 (UCP1) proteins in eWAT, while not affecting circulating irisin. VPA increased eWAT Tmem26 mRNA levels in the standard diet-fed group, whereas ET increased AMPK, interleukin 6 (IL-6) and fibronectin type III domain-containing protein 5 (FNDC5) protein expression in muscle, but had no impact on circulating irisin protein content. In eWAT, ET increased bone morphogenetic protein 7 (Bmp7), Cidea and PGC-1α in both diet-fed animals, whereas BMP7, Prdm16, UCP1 and FNDC5 only in standard diet-fed group.

SIGNIFICANCE:

Data suggest that ET-induced myokine production seems to contribute, at least in part, to the "brown-like" phenotype in WAT from rats fed a HFD.

KEYWORDS:

Adiposity; Browning; Endurance training; FNDC5/irisin; IL-6; Skeletal muscle; UCP1

PMID:
27693382
DOI:
10.1016/j.lfs.2016.09.023
[Indexed for MEDLINE]

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