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Curr Opin Biotechnol. 2017 Feb;43:62-69. doi: 10.1016/j.copbio.2016.09.003. Epub 2016 Sep 28.

Recent advancements in matrix-assisted laser desorption/ionization mass spectrometry imaging.

Author information

1
University of Victoria-Genome British Columbia Proteomics Centre, #3101-4464 Markham St., Vancouver Island Technology Park, Victoria, BC V8Z 7X8, Canada; Department of Biochemistry and Microbiology, University of Victoria, Petch Building Room 207, 3800 Finnerty Rd., Victoria, BC V8P 5C2, Canada.
2
University of Victoria-Genome British Columbia Proteomics Centre, #3101-4464 Markham St., Vancouver Island Technology Park, Victoria, BC V8Z 7X8, Canada.
3
University of Victoria-Genome British Columbia Proteomics Centre, #3101-4464 Markham St., Vancouver Island Technology Park, Victoria, BC V8Z 7X8, Canada; Department of Biochemistry and Microbiology, University of Victoria, Petch Building Room 207, 3800 Finnerty Rd., Victoria, BC V8P 5C2, Canada. Electronic address: christoph@proteincentre.com.

Abstract

Matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is a robust tool for spatially resolved analysis of biomolecules in situ. Recent advances in high ionization-efficiency MALDI matrices, new matrix deposition procedures, and the development of high spatial-resolution and high sensitivity MS instruments continue to drive new applications of MALDI-MSI, along with other MSI techniques, which allow us to visualize and determine the regio-specific and temporal changes in proteins, peptides, lipids, drug molecules, and metabolites within the tissues, cells and microorganisms. These provide researchers with a new route to the discovery of potential biomarkers of human disease and elucidation of the underlying biology of metabolic regulation, thus bringing our understanding of human health to a new level.

PMID:
27690313
DOI:
10.1016/j.copbio.2016.09.003
[Indexed for MEDLINE]

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