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Epigenomics. 2016 Nov;8(11):1481-1494. Epub 2016 Sep 30.

Characterizing the hypomethylated DNA methylation profile of nucleated red blood cells from cord blood.

Author information

1
Child & Family Research Institute, Vancouver, British Columbia, V5Z 4H4, Canada.
2
Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada.
3
Department of Pediatrics, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada.

Abstract

AIM:

To provide insight into fetal nucleated red blood cell (nRBC) development using genome-wide DNA methylation (DNAm) profiling.

MATERIALS & METHODS:

The DNAm profile (Illumina 450K array) of cord blood (n = 7) derived nRBCs was compared with B cells, CD4 and CD8 T cells, natural killer cells, granulocytes, monocytes and placenta (n = 5).

RESULTS:

nRBCs and placenta had similarly low array-wide DNAm compared with white blood cells, but their patterns of hypomethylation differed at biologically relevant subsets of the array. High interindividual variability in nRBC DNAm was driven by a negative association between DNAm and nRBC count.

CONCLUSION:

nRBC hypomethylation is likely an epigenetic signature of erythropoiesis rather than of early development. Variability in nRBC DNAm may stem from differences in the cell population's maturity or hematopoietic source.

KEYWORDS:

DNA methylation; Illumina 450K array; cord blood; epigenetics; nucleated red blood cells; placenta

PMID:
27687885
DOI:
10.2217/epi-2016-0069
[Indexed for MEDLINE]

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