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Proc Natl Acad Sci U S A. 2016 Oct 11;113(41):11414-11419. Epub 2016 Sep 28.

Effect of flow and peristaltic mixing on bacterial growth in a gut-like channel.

Author information

1
Department of Physics, University of California, San Diego, La Jolla, CA 92093-0374.
2
Department of Physics, University of California, San Diego, La Jolla, CA 92093-0374 agroisman@ucsd.edu hwa@ucsd.edu.

Abstract

The ecology of microbes in the gut has been shown to play important roles in the health of the host. To better understand microbial growth and population dynamics in the proximal colon, the primary region of bacterial growth in the gut, we built and applied a fluidic channel that we call the "minigut." This is a channel with an array of membrane valves along its length, which allows mimicking active contractions of the colonic wall. Repeated contraction is shown to be crucial in maintaining a steady-state bacterial population in the device despite strong flow along the channel that would otherwise cause bacterial washout. Depending on the flow rate and the frequency of contractions, the bacterial density profile exhibits varying spatial dependencies. For a synthetic cross-feeding community, the species abundance ratio is also strongly affected by mixing and flow along the length of the device. Complex mixing dynamics due to contractions is described well by an effective diffusion term. Bacterial dynamics is captured by a simple reaction-diffusion model without adjustable parameters. Our results suggest that flow and mixing play a major role in shaping the microbiota of the colon.

KEYWORDS:

bacterial growth; colon microbiota; in vitro gut model; peristalsis; reaction–diffusion model

PMID:
27681630
PMCID:
PMC5068270
DOI:
10.1073/pnas.1601306113
[Indexed for MEDLINE]
Free PMC Article

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