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Arch Pharm Res. 2016 Dec;39(12):1644-1652. doi: 10.1007/s12272-016-0840-7. Epub 2016 Sep 28.

P21 (Cdc42/Rac)-activated kinase 1 (pak1) is associated with cardiotoxicity induced by antihistamines.

Author information

1
Pharmacological Research Division, National Institute of Drug and Safety Evaluation, Ministry of Food and Drug Safety, Osong, Cheongju, 363-700, Republic of Korea. actpotyjs@korea.kr.
2
Pharmacological Research Division, National Institute of Drug and Safety Evaluation, Ministry of Food and Drug Safety, Osong, Cheongju, 363-700, Republic of Korea.
3
Pharmacological Research Division, National Institute of Drug and Safety Evaluation, Ministry of Food and Drug Safety, Osong, Cheongju, 363-700, Republic of Korea. kimhs58@korea.kr.

Abstract

Astemizole, a non-sedating histamine H1 receptor blocker, is widely known to cause cardiac arrhythmia, which prolongs the QT interval. However, the precise molecular mechanism involved in antihistamine-induced cardiovascular adverse effects other than hERG channel inhibition is still unclear. In this study, we used DNA microarray analysis to detect the mechanisms involved in life-threatening adverse effects caused by astemizole. Rat primary cardiomyocytes were treated with various concentrations of astemizole for 24 h and the corresponding cell lysates were analyzed using a DNA microarray. Astemizole altered the expression profiles of genes involved in calcium transport/signaling. Using qRT-PCR analysis, we demonstrated that, among those genes, p21 (Cdc42/Rac)-activated kinase 1 (pak1) mRNA was downregulated by treatment with terfenadine and astemizole. Astemizole also reduced pak1 protein levels in rat cardiomyocytes. In addition, astemizole decreased pak1 mRNA and protein levels in H9c2 cells and induced an increase in cell surface area (hypertrophy) and cytotoxicity. Fingolimod hydrochloride (FTY720), a pak1 activator, inhibited astemizole-induced hypertrophy and cytotoxicity in H9c2 cells. These results suggest that antihistamine-induced cardiac adverse effects are associated with pak1 expression and function.

KEYWORDS:

Antihistamine; Astemizole; Cardiotoxicity; Genomics

PMID:
27681411
DOI:
10.1007/s12272-016-0840-7
[Indexed for MEDLINE]

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