Format

Send to

Choose Destination
Plant Foods Hum Nutr. 2016 Dec;71(4):402-409.

Antioxidant and Anticancer Activities of Walnut (Juglans regia L.) Protein Hydrolysates Using Different Proteases.

Author information

1
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
2
Department of Food Science and Engineering, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran.
3
Department of Horticulture, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran.
4
Animal Biology Department, School of Biology, College of Sciences, University of Tehran, Tehran, Iran.
5
Departments of Ophthalmology and Visual Sciences, and Biomedical Engineering, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.
6
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran. moosavi@ut.ac.ir.
7
Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran. moosavi@ut.ac.ir.

Abstract

Walnut (Juglans regia L.) contains approximately 20-25 % protein with abundant essential amino acids. The enzymatic hydrolysate of Persian walnut (Chandler) seed proteins was prepared by incubation with three different proteases, including pancreatic chymotrypsin and trypsin, and a microbial enzyme proteinase K. The hydrolysates were found to possess excellent antioxidant capacities. The peptide fractions scavenged the 2, 2'-anizo-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) free radicals and inhibited the activity of reactive oxygen species. Walnut protein hydrolysates were also tested, for the first time, against the viability of human breast (MDA-MB231) and colon (HT-29) cancer cell lines. MTT, [3-(4, 5dimethylthiazolyl)-2,5-diphenyl-tetrazolium bromide], assay was used to assess in vitro cancer cell viability upon treatment with the peptide fractions. The peptide fractions showed cell growth inhibition of 63 ± 1.73 % for breast cancer and 51 ± 1.45 % for colon cancer cells. Thus, a direct correlation between antioxidant and anticancer activities of walnut peptide fractions exists and supports their potential therapeutic benefit.

KEYWORDS:

Anticancer; Antioxidant; Bioactive peptides; Protease; Walnut proteins

PMID:
27679440
PMCID:
PMC5223242
DOI:
10.1007/s11130-016-0576-z
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center