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World J Gastroenterol. 2016 Sep 7;22(33):7604-12. doi: 10.3748/wjg.v22.i33.7604.

Dried blood spots, valid screening for viral hepatitis and human immunodeficiency virus in real-life.

Author information

1
Belinda K Mössner, Benjamin Staugaard, Peer B Christensen, Department of Infectious Diseases, Odense University Hospital, 5000 Odense, Denmark.

Abstract

AIM:

To detect chronic hepatitis B (CHB), chronic hepatitis C (CHC) and human immunodeficiency virus (HIV) infections in dried blood spot (DBS) and compare these samples to venous blood sampling in real-life.

METHODS:

We included prospective patients with known viral infections from drug treatment centers, a prison and outpatient clinics and included blood donors as negative controls. Five drops of finger capillary blood were spotted on filter paper, and a venous blood sample was obtained. The samples were analyzed for HBsAg, anti-HBc, anti-HBs, anti-HCV, and anti-HIV levels as well as subjected to a combined nucleic acid test (NAT) for HBV DNA, HCV RNA and HIV RNA.

RESULTS:

Samples from 404 subjects were screened (85 CHB, 116 CHC, 114 HIV and 99 blood donors). DBS had a sensitivity of > 96% and a specificity of > 98% for the detection of all three infections. NAT testing did not improve sensitivity, but correctly classified 95% of the anti-HCV-positive patients with chronic and past infections. Anti-HBc and anti-HBS showed low sensitivity in DBS (68% and 42%).

CONCLUSION:

DBS sampling, combined with an automated analysis system, is a feasible screening method to diagnose chronic viral hepatitis and HIV infections outside of the health care system.

KEYWORDS:

Dried blood spot; Drug-users; Hepatitis B; Hepatitis C; Human immunodeficiency virus; People who inject drugs; Prisoners; Real-life; Screening

PMID:
27672281
PMCID:
PMC5011674
DOI:
10.3748/wjg.v22.i33.7604
[Indexed for MEDLINE]
Free PMC Article

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