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Nat Microbiol. 2016 Aug 1;1(10):16124. doi: 10.1038/nmicrobiol.2016.124.

Probing the metabolic heterogeneity of live Euglena gracilis with stimulated Raman scattering microscopy.

Author information

1
Department of Chemistry, University of Tokyo, Tokyo 113-0033, Japan.
2
Department of Electrical Engineering and Information Systems, University of Tokyo, Tokyo 113-8656, Japan.
3
Research &Development Department, Euglena Co., Ltd, Tokyo 108-0014, Japan.
4
Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, Japan.
5
Graduate School of Media and Governance, Keio University, Fujisawa, Kanagawa 252-8520, Japan.
6
Department of Information Processing and Computer Science, Chiba University, Chiba 263-8522, Japan.
7
Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.
8
Department of Electrical and Electronics Engineering, Chiba University, Chiba 263-8522, Japan.
9
Department of Electrical Engineering, University of California, Los Angeles, California 90095, USA.
10
Japan Science and Technology Agency, Tokyo 102-8666, Japan.

Abstract

Understanding metabolism in live microalgae is crucial for efficient biomaterial engineering, but conventional methods fail to evaluate heterogeneous populations of motile microalgae due to the labelling requirements and limited imaging speeds. Here, we demonstrate label-free video-rate metabolite imaging of live Euglena gracilis and statistical analysis of intracellular metabolite distributions under different culture conditions. Our approach provides further insights into understanding microalgal heterogeneity, optimizing culture methods and screening mutant microalgae.

PMID:
27670110
DOI:
10.1038/nmicrobiol.2016.124
[Indexed for MEDLINE]

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