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Bioorg Med Chem. 2016 Nov 1;24(21):5611-5617. doi: 10.1016/j.bmc.2016.09.022. Epub 2016 Sep 12.

Design and synthesis of novel HDAC8 inhibitory 2,5-disubstituted-1,3,4-oxadiazoles containing glycine and alanine hybrids with anti cancer activity.

Author information

1
GVK Biosciences Private Limited, IDA Mallapur, Hyderabad, Telangana 500076, India; Department of Biotechnology, Acharya Nagarjuna University, Guntur, Andhra Pradesh 522510, India. Electronic address: pvijayarao@gmail.com.
2
Department of Biochemistry/Bioinformatics, Institute of Science, GITAM University, Vishakhapatnam, Andhra Pradesh 530045, India.
3
PRIST University, Thanjavur 613 403, Tamil Nadu, India.
4
Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad, Telangana 500046, India.
5
Department of Biotechnology, Acharya Nagarjuna University, Guntur, Andhra Pradesh 522510, India. Electronic address: akrishnasatya78@gmail.com.

Abstract

Oxadiazole is a heterocyclic compound containing an oxygen atom and two nitrogen atoms in a five-membered ring. Of the four oxadiazoles known, 1,3,4-oxadiazole has become an important structural motif for the development of new drugs and the compounds containing 1,3,4-oxadiazole cores have a broad spectrum of biological activity. Herein, we describe the design, synthesis and biological evaluation of a series of novel 2,5-disubstituted 1,3,4-oxadiazoles (10a-10j) as class I histone deacetylase (HDAC) inhibitors. The compounds were designed and evaluated for HDAC8 selectivity using in silico docking software (Glide) and the top 10 compounds with high dock score and obeying Lipinski's rule were synthesized organically. Further the biological HDAC inhibitory and selectivity assays and anti-proliferative assays were carried out. In in silico and in vitro studies, all compounds (10a-10j) showed significant HDAC inhibition and exhibited HDAC8 selectivity. Among all tested compounds, 10b showed substantial HDAC8 inhibitory activity and better anticancer activity which is comparable to the positive control, a FDA approved drug, vorinostat (SAHA). Structural activity relation is discussed with various substitutions in the benzene ring connected on 1,3,4-oxadizole and glycine/alanine. The study warranted further investigations to develop HDAC8-selective inhibitory molecule as a drug for neoplastic diseases. Novel 1,3,4-oxadizole substituted with glycine/alanine showed HDAC8 inhibition.

KEYWORDS:

1,3,4-Oxadiazole; Alanine; Anti-proliferative effect; Class selective; Glycine; HDAC inhibition activity

PMID:
27665180
DOI:
10.1016/j.bmc.2016.09.022
[Indexed for MEDLINE]

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