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Food Chem. 2017 Feb 15;217:628-636. doi: 10.1016/j.foodchem.2016.09.021. Epub 2016 Sep 7.

Development of molecular imprinted column-on line-two dimensional liquid chromatography for selective determination of clenbuterol residues in biological samples.

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School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, PR China.
School of Pharmacy, Xi'an Jiaotong University, Xi'an 710061, PR China. Electronic address:


A novel method coupling molecular imprinted monolithic column with two-dimensional liquid chromatography was developed and validated for the analysis of clenbuterol in pork liver and swine urine samples. The polymers were characterized by using Fourier transform infrared spectroscopy, nitrogen adsorption desorption analyses, frontal analysis and the adsorption of selectivity. The results indicated that the imprinted columns were well prepared and possessed high selectivity adsorption capacity. Subsequently, the MIMC-2D-LC (molecular imprinted monolithic column-two dimensional liquid chromatography) method was developed for the selective analysis of clenbuterol in practical samples. The accuracy ranged from 94.3% to 99.7% and from 93.7% to 99.6% for liver and urine, respectively. The relative standard deviation (RSD) of repeatability was lower than 8.6% for both analyses. The limit of detections was 16ng·mL(-1) for liver and 25ng·mL(-1) for urine, respectively. Compared with the reported methods, the disturbance of endogenous impurity could be avoided by the 2D-LC method.


Clenbuterol; Molecular imprinted monolithic columns; Pork liver; Swine urine; Two-dimensional liquid chromatography

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