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Food Chem. 2017 Feb 15;217:505-510. doi: 10.1016/j.foodchem.2016.08.114. Epub 2016 Aug 29.

Comparison of gravimetric, creamatocrit and esterified fatty acid methods for determination of total fat content in human milk.

Author information

1
School of Chemistry and Biochemistry, The University of Western Australia, 35 Stirling Hwy, Crawley, Western Australia 6009, Australia. Electronic address: 21192741@student.uwa.edu.au.
2
School of Chemistry and Biochemistry, The University of Western Australia, 35 Stirling Hwy, Crawley, Western Australia 6009, Australia.
3
Separation Science and Metabolomics Laboratory, 90 South Street, Murdoch University, Murdoch, Western Australia 6150, Australia; Metabolomics Australia, Western Australia, Australia.
4
School of Chemistry and Biochemistry, The University of Western Australia, 35 Stirling Hwy, Crawley, Western Australia 6009, Australia. Electronic address: Donna.geddes@uwa.edu.au.

Abstract

The gravimetric method is considered the gold standard for measuring the fat content of human milk. However, it is labor intensive and requires large volumes of human milk. Other methods, such as creamatocrit and esterified fatty acid assay (EFA), have also been used widely in fat analysis. However, these methods have not been compared concurrently with the gravimetric method. Comparison of the three methods was conducted with human milk of varying fat content. Correlations between these methods were high (r(2)=0.99). Statistical differences (P<0.001) were observed in the overall fat measurements and within each group (low, medium and high fat milk) using the three methods. Overall, stronger correlation with lower mean (4.73g/L) and percentage differences (5.16%) was observed with the creamatocrit than the EFA method when compared to the gravimetric method. Furthermore, the ease of operation and real-time analysis make the creamatocrit method preferable.

KEYWORDS:

Creamatocrit; Esterified fatty acids; Fat content; Gravimetric; Human milk; Lactation

PMID:
27664665
DOI:
10.1016/j.foodchem.2016.08.114
[Indexed for MEDLINE]

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