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Obesity (Silver Spring). 2016 Nov;24(11):2368-2376. doi: 10.1002/oby.21655. Epub 2016 Sep 24.

MicroRNA-463-3p/ABCG4: A new axis in glucose-stimulated insulin secretion.

Author information

1
The Department of Human Anatomy of Jinzhou Medical University, Jinzhou, Liaoning, China.
2
School of Humanity and Management, Jinzhou Medical University, Jinzhou, Liaoning, China.
3
The Department of General Sugery, The First Affiliate Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China.
4
The Department of Human Anatomy of Jinzhou Medical University, Jinzhou, Liaoning, China. hxwily2015@sina.com.

Abstract

OBJECTIVE:

Glucose-stimulated insulin secretion (GSIS) is known to be essential in the control of metabolic fuel homeostasis, though the molecular mechanisms involved remain unclear.

METHODS:

MicroRNA (miRNA)-463-3p and ATP-binding cassette A4 (ABCG4) expression was analyzed by real-time PCR, and the potential role of miRNA-463-3p or ABCG4 was evaluated by overexpressing or silencing such miRNA or genes.

RESULTS:

The miRNA-463-3p inhibited GSIS without affecting cell viability. Further, mechanistic studies demonstrated that ABCG4 was a direct target of microRNA-463-3p and, to this effect, that ABCG4 played an important role in GSIS. The targeting was relevant in pancreatic islet β-cells, where GSIS through the miRNA-463-3p/ABCG4 axis was observed. Interestingly, in type 2 diabetes human pancreatic islets, expression of miRNA-463-3p and insulin was upregulated and ABCG4 downregulated compared with nondiabetic controls, and their expression levels were closely correlated.

CONCLUSIONS:

The findings collectively establish a link between GSIS and the miRNA-463-3p/ABCG4 axis and represent a promising target for future diabetes mellitus treatments.

PMID:
27664094
DOI:
10.1002/oby.21655
[Indexed for MEDLINE]
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