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Toxicol Lett. 2016 Nov 16;262:62-69. doi: 10.1016/j.toxlet.2016.09.005. Epub 2016 Sep 20.

Evaluation of the sensitizing potential of food proteins using two mouse models.

Author information

1
Institute for Risk Assessment Sciences, University Utrecht, Utrecht, The Netherlands; Utrecht Center for Food Allergy, Utrecht, The Netherlands. Electronic address: j.j.smit@uu.nl.
2
Utrecht Center for Food Allergy, Utrecht, The Netherlands; TNO, Zeist, The Netherlands.
3
Institute for Risk Assessment Sciences, University Utrecht, Utrecht, The Netherlands; Utrecht Center for Food Allergy, Utrecht, The Netherlands.

Abstract

The current methodology to identify allergenic food proteins is effective in identifying those that are likely to cross-react with known allergens. However, most assays show false positive results for low/non-allergens. Therefore, an ex vivo/in vitro DC-T cell assay and an in vivo mouse model were used to distinguish known allergenic food proteins (Ara h 1, β-Lactoglobulin, Pan b 1, bovine serum albumin, whey protein isolate) from low/non allergenic food proteins (soy lipoxygenase, gelatin, beef tropomyosin, rubisco, Sola t 1). CD4+ T cells from protein/alum-immunized mice were incubated with corresponding protein-pulsed bone marrow-derived DC and analyzed for cytokine release. All known allergens induced Th2 responses in vitro, whereas soy lipoxygenase, gelatin or beef tropomyosin did not. Sola t 1 and rubisco induced a more generalized T cell response due to endotoxin contamination, indicating the endotoxin-sensitivity of the DC-T assay. To analyze responses in vivo, mice were orally sensitized on days 0 and 7. Known allergens induced IgE and mMCP-1 release upon oral challenge at day 16, whereas the low/non-allergens did not. Both the DC-T cell assay and the mouse model were able to distinguish 5 known allergens from 5 low/non-allergens and may be useful to identify novel allergenic food proteins.

KEYWORDS:

Allergenic proteins; Allergenicity; Dendritic cells; Food allergy; Mouse models; Sensitization; T cells

PMID:
27663974
DOI:
10.1016/j.toxlet.2016.09.005
[Indexed for MEDLINE]

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