Manganese-induced cadmium stress tolerance in rice seedlings: Coordinated action of antioxidant defense, glyoxalase system and nutrient homeostasis

C R Biol. 2016 Nov-Dec;339(11-12):462-474. doi: 10.1016/j.crvi.2016.08.002. Epub 2016 Sep 20.

Abstract

The accumulation of cadmium (Cd) alters different physiological and biochemical attributes that affect plant growth and yield. In our study, we investigated the regulatory role of supplemental manganese (Mn) on hydroponically grown rice (Oryza sativa L. cv. BRRI dhan29) seedlings under Cd-stress conditions. Exposure of 14-d-old seedlings to 0.3mM CdCl2 for three days caused growth inhibition, chlorosis, nutrient imbalance, and higher Cd accumulation. Higher Cd uptake caused oxidative stress through lipid peroxidation, loss of plasma membrane integrity, and overproduction of reactive oxygen species (ROS) and methylglyoxal (MG). The exogenous application of 0.3mM MnSO4 to Cd-treated seedlings partly recovered Cd-induced water loss, chlorosis, growth inhibition, and nutrient imbalance by reducing Cd uptake and its further translocation to the upper part of the plant. Supplemental Mn also reduced Cd-induced oxidative damage and lipid peroxidation by improved antioxidant defense and glyoxalase systems through enhancing ROS and MG detoxification, respectively.

Keywords: Antioxidant defense glyoxalase system; Metal accumulation; Methylglyoxal; Oxidative damage; Reactive oxygen species; Trace elements.

MeSH terms

  • Antioxidants / pharmacology*
  • Cadmium / toxicity*
  • Chlorophyll / metabolism
  • Homeostasis / drug effects*
  • Lactoylglutathione Lyase / metabolism*
  • Lipid Peroxidation / drug effects
  • Manganese / pharmacology*
  • Nutritional Physiological Phenomena / drug effects*
  • Oryza / drug effects*
  • Oryza / growth & development
  • Oryza / metabolism
  • Oxidative Stress / drug effects
  • Pyruvaldehyde / metabolism
  • Reactive Oxygen Species / metabolism
  • Seedlings / drug effects
  • Water / metabolism

Substances

  • Antioxidants
  • Reactive Oxygen Species
  • Cadmium
  • Water
  • Chlorophyll
  • Manganese
  • Pyruvaldehyde
  • Lactoylglutathione Lyase