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Curr Opin Struct Biol. 2016 Dec;41:225-232. doi: 10.1016/j.sbi.2016.09.006. Epub 2016 Sep 21.

Single-molecule fluorescence microscopy of native macromolecular complexes.

Author information

1
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, USA.
2
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, USA; Department of Biophysics, Johns Hopkins University, USA; Department of Biomedical Engineering, Johns Hopkins University, USA; Howard Hughes Medical Institute, Baltimore, MD, USA.

Abstract

Macromolecular complexes consisting of proteins, lipids, and/or nucleic acids are ubiquitous in biological processes. Their composition, stoichiometry, order of assembly, and conformations can be heterogeneous or can change dynamically, making single-molecule studies best suited to measure these properties accurately. Recent single-molecule pull-down and other related approaches have combined the principles of conventional co-immunoprecipitation assay with single-molecule fluorescence microscopy to probe native macromolecular complexes. In this review, we present the advances in single-molecule pull-down methods and biological systems that have been investigated in such semi vivo manner.

PMID:
27662375
DOI:
10.1016/j.sbi.2016.09.006
[Indexed for MEDLINE]

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