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J Photochem Photobiol B. 2016 Nov;164:96-102. doi: 10.1016/j.jphotobiol.2016.09.017. Epub 2016 Sep 12.

Low-level laser therapy (904nm) can increase collagen and reduce oxidative and nitrosative stress in diabetic wounded mouse skin.

Author information

1
Physical Therapy Department, Federal University of Sao Carlos, Sao Carlos, SP, Brazil; Physical Therapy Department, Federal University of Ceara, Fortaleza, CE, Brazil; Department of Dermatology, Harvard Medical School, Boston, MA, USA; Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA. Electronic address: tatmatsu@gmail.com.
2
Physical Therapy Department, Federal University of Sao Carlos, Sao Carlos, SP, Brazil; Department of Dermatology, Harvard Medical School, Boston, MA, USA; Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA.
3
Department of Dermatology, Harvard Medical School, Boston, MA, USA; Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA.
4
Pharmacology Department, Federal University of Ceara, Fortaleza, CE, Brazil.
5
Ceara State University, Superior Institute of Biomedicine, Laboratory of Renal and Cardiovascular Pharmacology, Fortaleza, Ceará, Brazil.
6
Physical Therapy Department, Federal University of Sao Carlos, Sao Carlos, SP, Brazil.

Abstract

BACKGROUND AND OBJECTIVE:

Over the last decade we have seen an increased interest in the use of Low-Level Laser Therapy (LLLT) in diseases that involve increased oxidative stress. It is well established that hyperglycemia in diabetes elicits a rise in reactive oxygen species (ROS) production but the effect of LLLT remains unclear. This study aimed to investigate whether LLLT was able to improve oxidative/nitrosative stress parameters in the wound healing process in diabetic mice.

STUDY DESIGN/MATERIALS AND METHODS:

Twenty male mice were divided into four groups: non-irradiated control (NIC), irradiated control (IC), non-irradiated and diabetic (NID), irradiated and diabetic (ID). Diabetes was induced by administration of streptozotocin. Wounds were created 120days after the induction of diabetes in groups IC and ID and these groups were irradiated daily for 5days (superpulsed 904nm laser, average power 40mW, 60s). All animals were sacrificed 1day after the last irradiation and histology, collagen amount, catalase activity, nitrite and thiobarbituric acid reactive substances (TBARS) were measured.

RESULTS:

Histology showed that collagen fibers were more organized in IC and ID when compared to NID group, and significant differences in collagen content were found in group ID versus NID. Catalase activity was higher in IC group compared to other groups (p<0.001). TBARS levels were higher in IC versus NIC, but were lower in ID versus NID (p<0.001). Nitrite was lower in both irradiated groups versus the respective non-irradiated groups (p<0.001).

CONCLUSIONS:

Delayed wound healing in diabetes is still a challenge in clinical practice with high social costs. The increased production of collagen and decreased oxidative and nitrosative stress suggests that LLLT may be a viable therapeutic alternative in diabetic wound healing.

KEYWORDS:

Catalase; Collagen production; Diabetes mellitus; Nitric oxide; Oxidative stress; Photobiomodulation; TBARS

PMID:
27661759
PMCID:
PMC5087142
DOI:
10.1016/j.jphotobiol.2016.09.017
[Indexed for MEDLINE]
Free PMC Article

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