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J Periodontol. 2017 Feb;88(2):197-208. doi: 10.1902/jop.2016.160217. Epub 2016 Sep 23.

Benefits of Bifidobacterium animalis subsp. lactis Probiotic in Experimental Periodontitis.

Author information

1
Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry of Ribeirao Preto, University of São Paulo, São Paulo, Brazil.
2
Department of Clinical Analyses, School of Pharmaceutical Sciences of Ribeirao Preto, University of São Paulo.
3
Department of Periodontology, Dental Research Division, Guarulhos University, São Paulo, Brazil.
4
Department of Prosthodontics and Periodontics, School of Dentistry, Campinas State University, São Paulo, Brazil.
5
Department of Basic Sciences, Division of Histology, Dental School of Aracatuba, São Paulo State University, São Paulo, Brazil.

Abstract

BACKGROUND:

This study evaluates effects of topical administration of probiotic bacteria of the genus Bifidobacterium on experimental periodontitis (EP) in rats.

METHODS:

Thirty-two rats were divided into groups C (control; without EP), EP (EP only), C-HN019 (control+probiotic), and EP-HN019 (EP+probiotic). On day 0 of the experiment, animals of groups EP and EP-HN019 received cotton ligatures around mandibular first molars (MFMs). In groups C-HN019 and EP-HN019, 1 mL of suspensions containing Bifidobacterium animalis subsp. lactis (B. lactis) HN019 was topically administered in the subgingival region of MFMs on days 0, 3, and 7. In groups C and EP, topical administrations were performed using a sham suspension (without probiotic). All animals were euthanized at day 14. Gingival tissue, hemimandibles, and oral biofilm were collected. Data were statistically analyzed (P <0.05).

RESULTS:

Group EP presented greater bone porosity, trabecular separation, and connective tissue attachment loss (CTAL) as well as reduced bone volume than all other groups (P <0.05). In group EP-HN019, there were greater proportions of Actinomyces and Streptococcus-like species and lower proportions of Veillonella parvula, Capnocytophaga sputigena, Eikenella corrodens, and Prevotella intermedia-like species than group EP. Group EP-HN019 presented greater expressions of osteoprotegerin and β-defensins than group EP (P <0.05). Group EP presented greater levels of interleukin-1β and receptor activator of nuclear factor-kappa B ligand than group EP-HN019 (P <0.05).

CONCLUSION:

Topical use of B. lactis HN019 promotes a protective effect against alveolar bone loss and CTALs attributable to EP in rats, modifying immunoinflammatory and microbiologic parameters.

KEYWORDS:

Alveolar bone loss; Bifidobacterium; periodontal attachment loss; periodontitis; probiotics; rats

PMID:
27660886
DOI:
10.1902/jop.2016.160217
[Indexed for MEDLINE]

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