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Am J Physiol Gastrointest Liver Physiol. 2016 Nov 1;311(5):G880-G894. doi: 10.1152/ajpgi.00250.2016. Epub 2016 Sep 22.

Imaging activation of peptidergic spinal afferent varicosities within visceral organs using novel CGRPα-mCherry reporter mice.

Author information

1
Discipline of Human Physiology and Centre for Neuroscience, School of Medicine, Flinders University of South Australia, Adelaide, South Australia, Australia nicholas.spencer@flinders.edu.au.
2
Discipline of Human Physiology and Centre for Neuroscience, School of Medicine, Flinders University of South Australia, Adelaide, South Australia, Australia.

Abstract

In vertebrates, visceral pain from internal organs is detected by spinal afferents, whose cell bodies lie in dorsal root ganglia (DRG). Until now, all recordings from spinal afferents have been restricted to recording transmission of action potentials along axons, or from cell bodies lying outside their target organ, which is not where sensory transduction occurs. Our aim was to record directly from a major class of spinal afferent within visceral organs, where transduction of sensory stimuli into action potentials occurs. Using novel calcitonin gene-related peptide (CGRP)α reporter mice, DRG neurons expressed mCherry, including nerve axons within viscera. In colon, a minority of total CGRP immunoreactivity was attributed CGRPα. In isolated unstretched colon, calcium imaging from CGRPα-expressing varicose axons did not detect resolvable calcium transients. However, noxious levels of maintained circumferential stretch to the colon induced repetitive calcium transients simultaneously in multiple neighboring varicosities along single mCherry-expressing axons. Discrete varicosities could generate unitary calcium transients independently of neighboring varicosities. However, axons expressing mCherry only generated coordinated calcium transients when accompanied by simultaneous activation of multiple varicosities along that axon. Simultaneous imaging from different classes of myenteric neurons at the same time as mCherry-expressing axons revealed coordinated calcium transients in multiple myenteric neurons, independent of activity in mCherry-expressing axons. CGRPα-expressing axon terminals preferentially responded to heat, capsaicin, and low pH. We show that direct recordings can be made from the major class of peptidergic spinal afferent that contributes to visceral nociception. This approach can provide powerful insights into transduction of stimuli in viscera.

KEYWORDS:

calcium imaging; colon; nociception; pain; spinal afferent

PMID:
27659421
DOI:
10.1152/ajpgi.00250.2016
[Indexed for MEDLINE]
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