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Respir Res. 2016 Sep 22;17(1):118.

Discordance in cathepsin B and cystatin C expressions in bronchoalveolar fluids between murine bleomycin-induced fibrosis and human idiopathic fibrosis.

Author information

  • 1INSERM, UMR 1100/Centre d'Etude des Pathologies Respiratoires (CEPR), Université François Rabelais, Faculté de Médecine, 10 Boulevard Tonnellé, F-37032, Tours, cedex, France.
  • 2Present address: Laboratoire de Biologie des Tumeurs et du Développement (LBTD), Université de Liège, Liège, Belgium.
  • 3CNRS, UMR-INEM 7355, Immunologie et Neurogénétique Experimentales et Moléculaires, Université d'Orléans, Orléans, France.
  • 4Institute of Molecular Medicine and Cell Research, Medical Faculty, Albert-Ludwigs-University Freiburg, Freiburg, Germany.
  • 5Centre Hospitalier Régional Universitaire Tours, Service de Pneumologie, Tours, France.
  • 6INSERM, UMR 1100/Centre d'Etude des Pathologies Respiratoires (CEPR), Université François Rabelais, Faculté de Médecine, 10 Boulevard Tonnellé, F-37032, Tours, cedex, France. gilles.lalmanach@univ-tours.fr.

Abstract

The activity of cysteine cathepsin B increased markedly in lung homogenates and in bronchoalveolar lavage fluids (BALF) of the mouse model of bleomycin-induced lung fibrosis after 14 days of challenge. In contrast the level of the cysteine cathepsin inhibitor cystatin C was unaffected in BALF of wild-type and cathepsin B-deficient mice. Therefore, murine cystatin C is not a reliable marker of fibrosis during bleomycin-induced lung fibrosis. Current data are in sharp contrast to previous analysis carried on human BALF from patients with idiopathic pulmonary fibrosis, for which the level of cathepsin B remained unchanged while cystatin C was significantly increased.

KEYWORDS:

Bleomycin; Bronchoalveolar lavage fluid; Cathepsins; Cystatins; Fibrosis; Lung; Proteases

PMID:
27658724
PMCID:
PMC5034538
DOI:
10.1186/s12931-016-0432-6
[PubMed - as supplied by publisher]
Free PMC Article

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