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Genetics. 2016 Nov;204(3):1065-1074. doi: 10.1534/genetics.116.194696. Epub 2016 Sep 21.

Donor Preference Meets Heterochromatin: Moonlighting Activities of a Recombinational Enhancer in Saccharomyces cerevisiae.

Author information

1
Department of Molecular and Cell Biology, California Institute for Quantitative Biosciences, University of California, Berkeley, California 94720-3220.
2
Department of Molecular and Cell Biology, California Institute for Quantitative Biosciences, University of California, Berkeley, California 94720-3220 jrine@berkeley.edu.

Abstract

In Saccharomyces cerevisiae, a small, intergenic region known as the recombination enhancer regulates donor selection during mating-type switching and also helps shape the conformation of chromosome III. Using an assay that detects transient losses of heterochromatic repression, we found that the recombination enhancer also acts at a distance in cis to modify the stability of gene silencing. In a mating-type-specific manner, the recombination enhancer destabilized the heterochromatic repression of a gene located ∼17 kbp away. This effect depended on a subregion of the recombination enhancer that is largely sufficient to determine donor preference. Therefore, this subregion affects both recombination and transcription from a distance. These observations identify a rare example of long-range transcriptional regulation in yeast and raise the question of whether other cis elements also mediate dual effects on recombination and gene expression.

KEYWORDS:

Sas2; long-range regulation; mating-type switching; silencing

PMID:
27655944
PMCID:
PMC5105842
DOI:
10.1534/genetics.116.194696
[Indexed for MEDLINE]
Free PMC Article

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