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Braz Dent J. 2016 Jul-Aug;27(4):404-7. doi: 10.1590/0103-6440201600748.

Fibroblast Viability after Storage at 20 °C in Milk, Hank's Balanced Salt Solution and Coconut Water.

Author information

1
Department of Dentistry, UFSC - Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
2
Doctoral Program in Dentistry, UFSC - Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
3
Department of Pharmaceutical Sciences, UFSC - Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.

Abstract

The objective of this study was to evaluate the effectiveness of various storage media at 20 °C in maintaining the viability of human periodontal ligament fibroblasts (HPLF) over time. HPLF were maintained at 20 °C in skim milk (SM), whole milk (WM), freshly prepared Hank's balanced salt solution (HBSS), Save-A-Tooth(r), natural coconut water (NCW), coconut water industrialized (ICW) and tap water (negative control) for 3, 6, 24, 48, 72, 96 and 120 h. Cells maintained in Minimal Essential Medium (MEM-37) at 37 °C served as a positive control. Cell viability was determined by MTT assay. Statistical analysis was performed by Kruskal-Wallis test and Scheffe test (α = 5%). From 24 h, NCW was significantly better in maintaining cell viability than all other tested storage media (p<0.05). SM and WM were significantly better than HBSS for up to 72 h. Save-A-Tooth(r) and ICW were the worst conservation storage media. In conclusion, the effectiveness of the tested storage media to maintain the viability of the periodontal ligament cells was as follows, in a descending order: NCW > MEM-37> SM and IM> HBSS> ICW > Save-A-Tooth(r)> tap water.

PMID:
27652701
DOI:
10.1590/0103-6440201600748
[Indexed for MEDLINE]
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