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J Mol Histol. 2016 Dec;47(6):541-554. Epub 2016 Sep 20.

N-cadherin regulates beta-catenin signal and its misexpression perturbs commissural axon projection in the developing chicken spinal cord.

Yang C1,2, Li X1, Wang C2, Fu S1, Li H1,3, Guo Z2, Zhao S4, Lin J5,6,7.

Author information

1
College of Life Science and Technology, Xinxiang Medical University, Xinxiang, 453003, China.
2
Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang, 453003, China.
3
Advanced Medical and Dental Institute, Universiti Sains Malaysia, 13200, Bertam, Penang, Malaysia.
4
College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China.
5
College of Life Science and Technology, Xinxiang Medical University, Xinxiang, 453003, China. developmentlab@126.com.
6
Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang, 453003, China. developmentlab@126.com.
7
Institute of Anatomy I, Jena University Hospital, 07743, Jena, Germany. developmentlab@126.com.

Abstract

N-cadherin is a calcium-sensitive cell adhesion molecule that plays an important role in the formation of the neural circuit and the development of the nervous system. In the present study, we investigated the function of N-cadherin in cell-cell connection in vitro with HEK293T cells, and in commissural axon projections in the developing chicken spinal cord using in ovo electroporation. Cell-cell connections increased with N-cadherin overexpression in HEK293T cells, while cell contacts disappeared after co-transfection with an N-cadherin-shRNA plasmid. The knockdown of N-cadherin caused the accumulation of β-catenin in the nucleus, supporting the notion that N-cadherin regulates β-catenin signaling in vitro. Furthermore, N-cadherin misexpression perturbed commissural axon projections in the spinal cord. The overexpression of N-cadherin reduced the number of axons that projected alongside the contralateral margin of the floor plate, and formed intermediate longitudinal commissural axons. In contrast, the knockdown of N-cadherin perturbed commissural axon projections significantly, affecting the projections alongside the contralateral margin of the floor plate, but did not affect intermediate longitudinal commissural axons. Taken together, these findings suggest that N-cadherin regulates commissural axon projections in the developing chicken spinal cord.

KEYWORDS:

Axon projection; Beta-catenin signaling; Cell connection; In vivo electroporation; N-cadherin

PMID:
27650519
DOI:
10.1007/s10735-016-9698-8
[Indexed for MEDLINE]

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