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Plant Cell. 2016 Oct;28(10):2616-2631. Epub 2016 Sep 20.

Transcriptional Regulation of Arabidopsis Polycomb Repressive Complex 2 Coordinates Cell-Type Proliferation and Differentiation.

Author information

1
Department of Plant Biology and Genome Center, University of California, Davis, California 95616.
2
Institut de Biologie de l'Ecole Normale Supérieure, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8197, Institut National de la Santé et de la Recherche Médicale, U1024 Paris, France.
3
RIKEN Center for Sustainable Resource Science, Tsurumi, Yokohama 230-0045, Japan.
4
Department of Plant Biology and Genome Center, University of California, Davis, California 95616 sbrady@ucdavis.edu.

Abstract

Spatiotemporal regulation of transcription is fine-tuned at multiple levels, including chromatin compaction. Polycomb Repressive Complex 2 (PRC2) catalyzes the trimethylation of Histone 3 at lysine 27 (H3K27me3), which is the hallmark of a repressive chromatin state. Multiple PRC2 complexes have been reported in Arabidopsis thaliana to control the expression of genes involved in developmental transitions and maintenance of organ identity. Here, we show that PRC2 member genes display complex spatiotemporal gene expression patterns and function in root meristem and vascular cell proliferation and specification. Furthermore, PRC2 gene expression patterns correspond with vascular and nonvascular tissue-specific H3K27me3-marked genes. This tissue-specific repression via H3K27me3 regulates the balance between cell proliferation and differentiation. Using enhanced yeast one-hybrid analysis, upstream regulators of the PRC2 member genes are identified, and genetic analysis demonstrates that transcriptional regulation of some PRC2 genes plays an important role in determining PRC2 spatiotemporal activity within a developing organ.

PMID:
27650334
PMCID:
PMC5134969
DOI:
10.1105/tpc.15.00744
[Indexed for MEDLINE]
Free PMC Article

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