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Nutr Neurosci. 2018 Feb;21(2):97-107. doi: 10.1080/1028415X.2016.1222332. Epub 2016 Sep 20.

Agaricus blazei extract attenuates rotenone-induced apoptosis through its mitochondrial protective and antioxidant properties in SH-SY5Y neuroblastoma cells.

Author information

1
a Department of Anatomy , Bharath University , Selaiyur, Chennai , Tamil Nadu 600073 , India.
2
b Department of Anatomy , Velammal Medical College and Hospital , Madurai , Tamil Nadu 625009 , India.
3
c Department of Biochemistry and Biotechnology , Annamalai University , Annamalainagar , Tamil Nadu 608002 , India.
4
d Department of Food Science and Nutrition, CAMS , Sultan Qaboos University , Muscat , Oman.
5
e Ageing and Dementia Research Group , Sultan Qaboos University , Muscat , Oman.
6
f Food and Brain Research Foundation , Chennai , Tamil Nadu 600094 , India.
7
g Department of Radiology , Sri Lakshminarayana Institute of Medical Sciences , Puducherry , India.

Abstract

The present study was aimed to find out the effect of Agaricus blazei mushroom extract against rotenone-induced cellular model. SH-SY5Y neuroblastoma cells are divided into four experimental groups (control, rotenone (100 nM), A. blazei (5 μg/ml) + rotenone (100 nM), and A. blazei alone treated) based on MTT assay, cells were allowed to measure the ROS, TBARS levels, and antioxidants activities. Finally, mitochondrial transmembrane potential (MMP) and expressions of apoptotic proteins were also analyzed. Pre-treatment with A. blazei significantly enhanced cell viability, attenuated rotenone-induced ROS, MMP, and apoptosis. Our results indicated that anti-apoptotic properties of this natural compound due to its antioxidant and mitochondrial protective function protect rotenone-induced cytotoxicity. Therefore, it may be concluded that A. blazei can be further developed as a promising drug for the treatment of Parkinson's disease (PD).

KEYWORDS:

A. blazei; Apoptosis; Mitochondria membrane potential; Oxidative stress; Rotenone

PMID:
27646574
DOI:
10.1080/1028415X.2016.1222332
[Indexed for MEDLINE]

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