Format

Send to

Choose Destination
Matrix Biol. 2017 Jul;60-61:206-216. doi: 10.1016/j.matbio.2016.09.007. Epub 2016 Sep 17.

Hyaluronic acid-laminin hydrogels increase neural stem cell transplant retention and migratory response to SDF-1α.

Author information

1
School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, United States.
2
Department of Biomedical Engineering, New Jersey Institute of Technology, Newark, NJ, United States.
3
School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, United States; Barrow Brain Tumor Research Center, Barrow Neurological Institute, Phoenix, AZ, United States.
4
School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, United States. Electronic address: sarah.stabenfeldt@asu.edu.

Abstract

The chemokine SDF-1α plays a critical role in mediating stem cell response to injury and disease and has specifically been shown to mobilize neural progenitor/stem cells (NPSCs) towards sites of neural injury. Current neural transplant paradigms within the brain suffer from low rates of retention and engraftment after injury. Therefore, increasing transplant sensitivity to injury-induced SDF-1α represents a method for increasing neural transplant efficacy. Previously, we have reported on a hyaluronic acid-laminin based hydrogel (HA-Lm gel) that increases NPSC expression of SDF-1α receptor, CXCR4, and subsequently, NPSC chemotactic migration towards a source of SDF-1α in vitro. The study presented here investigates the capacity of the HA-Lm gel to promote NPSC response to exogenous SDF-1α in vivo. We observed the HA-Lm gel to significantly increase NPSC transplant retention and migration in response to SDF-1α in a manner critically dependent on signaling via the SDF-1α-CXCR4 axis. This work lays the foundation for development of a more effective cell therapy for neural injury, but also has broader implications in the fields of tissue engineering and regenerative medicine given the essential roles of SDF-1α across injury and disease states.

KEYWORDS:

Biomaterials; CXCL12; Chemotaxis; Neural progenitor cells; Regenerative medicine; Tissue engineering

PMID:
27645115
PMCID:
PMC5357205
DOI:
10.1016/j.matbio.2016.09.007
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center