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Nat Methods. 2016 Nov;13(11):919-922. doi: 10.1038/nmeth.3999. Epub 2016 Sep 19.

HiChIP: efficient and sensitive analysis of protein-directed genome architecture.

Author information

1
Center for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, California, USA.
2
Program in Epithelial Biology, Stanford University School of Medicine, Stanford, California, USA.
3
Department of Genetics, Stanford University School of Medicine, Stanford, California, USA.
4
Department of Applied Physics, Stanford University, Stanford, California, USA.

Abstract

Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the yield of conformation-informative reads by over 10-fold and lowers the input requirement over 100-fold relative to that of ChIA-PET. HiChIP of cohesin reveals multiscale genome architecture with greater signal-to-background ratios than those of in situ Hi-C.

PMID:
27643841
PMCID:
PMC5501173
DOI:
10.1038/nmeth.3999
[Indexed for MEDLINE]
Free PMC Article

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