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Life Sci. 2016 Nov 15;165:56-62. doi: 10.1016/j.lfs.2016.09.010. Epub 2016 Sep 15.

Comparing the effects of endogenous and synthetic cannabinoid receptor agonists on survival of gastric cancer cells.

Author information

1
Laboratorio de Medicina Translacional, Instituto Nacional de Cancerología, SSA. Mexico City 14080, Mexico.
2
Unidad de Oncología Torácica, Instituto Nacional de Cancerología, SSA. Mexico City 14080, Mexico.
3
Laboratorio de Cannabinoides, Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de Mexico City, 04510, Mexico.
4
Laboratorio de Aminoácidos Excitadores, Instituto Nacional de Neurología y Neurocirugía, SSA. Mexico City 14269, Mexico. Electronic address: absada@yahoo.com.

Abstract

AIMS:

Anti-neoplastic activity induced by cannabinoids has been extensively documented for a number of cancer cell types; however, this topic has been explored in gastric cancer cells only in a limited number of approaches. Thus, the need of integrative and comparative studies still persists.

MATERIALS AND METHODS:

In this study we tested and compared the effects of three different cannabinoid receptor agonists-anandamide (AEA), (R)-(+)-methanandamide (Meth-AEA) and CP 55,940 (CP)- on gastric cancer cell morphology, viability and death events in order to provide new insights to the use of these agents for therapeutic purposes.

KEY FINDINGS:

The three agents tested exhibited similar concentration-dependent effects in the induction of changes in cell morphology and cell loss, as well as in the decrease of cell viability and DNA laddering in the human gastric adenocarcinoma cell line (AGS). Differences among the cannabinoids tested were mostly observed in the density of cells found in early and late apoptosis and necrosis, favoring AEA and CP as the more effective inducers of apoptotic mechanisms, and Meth-AEA as a more effective inducer of necrosis through transient and rapid apoptosis.

SIGNIFICANCE:

Through a comparative approach, our results support and confirm the therapeutic potential that cannabinoid receptor agonists exert in gastric cancer cells and open possibilities to use cannabinoids as part of a new gastric cancer therapy.

KEYWORDS:

Anti-proliferative activity; Anti-tumor therapy; Apoptosis; Cannabinoid receptor agonists; Gastric cancer cells

PMID:
27640887
DOI:
10.1016/j.lfs.2016.09.010
[Indexed for MEDLINE]

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