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Life Sci. 2016 Nov 15;165:16-20. doi: 10.1016/j.lfs.2016.09.009. Epub 2016 Sep 15.

dl-3-n-Butylphthalide attenuation of methamphetamine-induced neurotoxicity in SH-SY5Y neuroblastoma cells.

Author information

1
Department of Neurology, the Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China; Department of Neurology, Taian City Central Hospital, Taian 271000, China.
2
Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68105, USA.
3
Department of Neurology, Taian City Central Hospital, Taian 271000, China.
4
Department of Neurology, the Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China. Electronic address: xieanmu@163.com.
5
Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE 68105, USA. Electronic address: hxiong@unmc.edu.

Abstract

AIMS:

Methamphetamine (Meth) abuse causes neural injury in the brain. There are no efficacious therapies available to treat Meth-induce neural injury. The present study intended to test the therapeutic potential of dl-3-n-butylphthalide (NBP), a chemical compound extracted originally from the seeds of Chinese Celery, in the amelioration and prevention of Meth-induced neural injury.

MAIN METHODS:

Experiments were carried out on SH-SY5Y cells. Neuronal injury and apoptotic cell death were detected by MTT assay and analysis of nuclear morphology. Intracellular reactive oxygen species (ROS) was evaluated by dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay and protein expression levels of the apoptosis-related cleaved caspase-3, bcl2 and Bax were determined by western blotting.

KEY FINDINGS:

Treatment of SH-SY5Y cells with Meth induced cell injury and apoptosis. NBP attenuated Meth-associated cell injury and apoptosis via blockage of Meth-mediated upregulation of intracellular ROS production and inhibition of Meth-induced decrease of cleaved caspase-3/caspase-3 and Bcl-2/Bax ratios.

SIGNIFICANCE:

The results presented in this study indicate that NBP may have therapeutic benefits in the treatment of Meth-induced neuronal injury in the central nervous system.

KEYWORDS:

Methamphetamine; Neuroprotection; Neurotoxicity; dl-3-n-Butylphthalide

PMID:
27640886
DOI:
10.1016/j.lfs.2016.09.009
[Indexed for MEDLINE]

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