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Fish Shellfish Immunol. 2016 Nov;58:259-265. doi: 10.1016/j.fsi.2016.09.031. Epub 2016 Sep 15.

Identification and expressional analysis of NLRC5 inflammasome gene in smolting Atlantic salmon (Salmo salar).

Author information

1
Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile; Programa Doctorado en Ciencias, Mención Biología Celular y Molecular, Escuela de Graduados, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile. Electronic address: jppontigo@gmail.com.
2
Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile.
3
Instituto de Bioquímica y Microbiología, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile.
4
Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile; Programa de Doctorado en Ciencias de la Acuicultura, Universidad Austral de Chile, Los Pinos s/n, Balneario Pelluco, Puerto Montt, Chile.
5
Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile; Programa de Magister en Ciencias, Mención Microbiología, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile.
6
Programa de Doctorado en Ciencias de la Acuicultura, Universidad Austral de Chile, Los Pinos s/n, Balneario Pelluco, Puerto Montt, Chile; Marine Harvest Chile, Ruta 226 Km8, Puerto Montt, Chile.
7
Trapananda Asesorias, Cristobal Colon 486, Puerto Montt, Chile.
8
Instituto de Farmacología y Morfofisiología, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile.
9
Instituto de Bioquímica y Microbiología, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile; Interdisciplinary Center for Aquaculture Research (INCAR), Concepción, Chile. Electronic address: ayanez@uach.cl.
10
Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile; Centro Fondap de Investigación de Altas Latitudes (IDEAL), Universidad Austral deChile, Casilla 567, Valdivia, Chile. Electronic address: luis.vargas@uach.cl.

Abstract

The NOD-like receptors (NLRs) were recently identified as an intracellular pathogen recognition receptor family in vertebrates. While the immune system participation of NLRs has been characterized and analyzed in various mammalian models, few studies have considered NLRs in teleost species. Therefore, this study analyzed the Atlantic salmon (Salmo salar) NLRC5. Structurally, Atlantic salmon NLRC5 presented leucine-rich repeat subfamily genes. Phylogenetically, NLRC5 was moderately conserved between S. salar and other species. Real-time quantitative PCR revealed NLRC5 expression in almost all analyzed organs, with greatest expressions in the head kidney, spleen, and hindgut. Furthermore, NLRC5 gene expression decreased during smolt stage. These data suggest that NLRC5 participates in the Atlantic salmon immune response and is regulated, at least partly, by the smoltification process, suggesting that there is a depression of immune system from parr at smolt stage. This is the first report on the NLRC5 gene in salmonid smolts.

KEYWORDS:

Inflammation; Innate immunity; Leucine-rich repeats; NLR; NOD-like receptors; Nucleotide binding; Pathogen

PMID:
27640334
DOI:
10.1016/j.fsi.2016.09.031
[Indexed for MEDLINE]

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