Format

Send to

Choose Destination
Diabetes Res Clin Pract. 2016 Nov;121:41-48. doi: 10.1016/j.diabres.2016.08.019. Epub 2016 Sep 8.

Association of aldose reductase gene (AKR1B1) polymorphism with diabetic retinopathy.

Author information

1
Department of Human Genetics, Guru Nanak Dev University, Amritsar, Punjab, India. Electronic address: navdeepsidhu2490@gmail.com.
2
Department of Human Genetics, Guru Nanak Dev University, Amritsar, Punjab, India. Electronic address: vanita_kumar@yahoo.com.

Abstract

AIMS:

Present study aimed to investigate the association of aldose reductase (AKR1B1) gene polymorphism (-106C>T; rs759853) with diabetic retinopathy (DR) in type 2 diabetes mellitus (T2DM) patients from north India.

METHODS:

The present case-control association study recruited 926 subjects, including 487 DR patients and 439 individuals with confirmed T2DM as controls (CDR). AKR1B1 -106C>T polymorphism analysis in these 926 subjects was performed by polymerase chain reaction and direct DNA sequence analysis. Statistical analysis was performed using SPSS package.

RESULTS:

Statistically significant differences were observed between the two analyzed groups in the age of onset of diabetes (p=0.000) and duration of diabetes (p=0.000). Genotype distribution of AKR1B1 -106C>T polymorphism differed significantly between DR and CDR groups (p=0.02), however, distribution of allele frequency did not differ significantly (p=0.19). Binary logistic regression analyses showed an association of homozygous recessive TT genotype with diabetic retinopathy (OR: 1.61%, 95% CI, 1.39-2.284, p<0.01) in comparison to wild type CC genotype.

CONCLUSIONS:

These findings suggest a statistically significant association of AKR1B1 -106C>T polymorphism with retinopathy in North Indian patients. To our knowledge, this is the first report of association of -106C>T polymorphism in AKR1B1 in DR patients from India.

KEYWORDS:

-106C>T polymorphism; AKR1B1; Case-control association study; DNA sequencing; Diabetic retinopathy; Type 2 diabetes

PMID:
27640118
DOI:
10.1016/j.diabres.2016.08.019
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center