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J Assist Reprod Genet. 2016 Dec;33(12):1685-1690. Epub 2016 Sep 17.

Developmental ability of embryos produced from oocytes with fragile oolemma by intracytoplasmic sperm injection.

Author information

1
Aiiku Ladies Clinic, 1-40-2 Komatsubara, Kagoshima, 891-0114, Japan. lca.aiiku.lab.3f@gmail.com.
2
The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan. lca.aiiku.lab.3f@gmail.com.
3
Aiiku Ladies Clinic, 1-40-2 Komatsubara, Kagoshima, 891-0114, Japan.
4
The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan.

Abstract

PURPOSE:

In intracytoplasmic sperm injection (ICSI) of oocytes with a fragile oolemma (fragile oocytes), breakage can occur at injection. In this study, we produced embryos from oocytes with a fragile and normal oolemma (normal oocytes) by ICSI and compared their ability to be fertilized and develop in vitro. We also investigated whether fragile oocyte-derived embryos could implant after blastocyst transfer to determine whether fragile oocytes should be used for assisted reproductive technology treatment.

METHODS:

Oocytes were divided into three groups-normal oocytes from cycles containing no fragile oocytes (group A), normal oocytes from cycles containing at least one fragile oocyte (group B), and fragile oocytes (group C), and their fertilization abilities after ICSI and the developmental abilities of resultant embryos were compared.

RESULTS:

The fertilization rate in group C (65.3 %) was significantly (P < 0.01) lower than those in groups A (84.6 %) and B (86.9 %), and the degeneration rate in group C (24.2 %) was significantly (P < 0.01) higher than those in groups A (0.71 %) and B (0.28 %). However, there were no significant differences in the blastocyst formation rates (59.7-67.5 %) of embryos among the different groups. In addition, the pregnancy rate after transfer of blastocysts in group C (50.0 %) was not significantly different from those in groups A (35.6 %) and B (45.8 %).

CONCLUSIONS:

The fertilization ability after ICSI of fragile oocytes is lower than that of normal oocytes but the resultant embryos have the same developmental ability as those of normal oocyte-derived embryos.

KEYWORDS:

Embryo; In vitro fertilization; Intracytoplasmic sperm injection; Oocyte plasma membrane; Oolemma; Time-lapse incubator

PMID:
27639997
PMCID:
PMC5171884
DOI:
10.1007/s10815-016-0811-4
[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

The authors declare that they have no conflict of interest. Research involving human participants This study was approved by the Institutional Review Board of AIIKUKAI Medical Corporation. Informed consent Written informed consent for their treatment and for their outcomes to be described was obtained from all patients.

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