Format

Send to

Choose Destination
Bioresour Technol. 2016 Dec;221:165-171. doi: 10.1016/j.biortech.2016.09.004. Epub 2016 Sep 4.

The performance of microbial anodes in municipal wastewater: Pre-grown multispecies biofilm vs. natural inocula.

Author information

1
Laboratory for MEMS Applications, IMTEK - Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany.
2
Institute for Applied Biosciences, Department of Applied Biology, Karlsruhe Institute of Technology, Fritz-Haber-Weg 2, 76131 Karlsruhe, Germany.
3
Institute for Applied Biosciences, Department of Applied Biology, Karlsruhe Institute of Technology, Fritz-Haber-Weg 2, 76131 Karlsruhe, Germany; Institute for Biological Interfaces, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, D-76344 Eggenstein-Leopoldshafen, Germany.
4
Laboratory for MEMS Applications, IMTEK - Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany. Electronic address: kerzenma@imtek.uni-freiburg.de.

Abstract

In this study, different inoculation strategies for continuously operated microbial anodes are analyzed and compared. After 20daysof operation with municipal wastewater anodes pre-incubated with a biofilm of the exoelectrogenic species Geobacter and Shewanella showed current densities of (65±8) μA/cm2. This is comparable to the current densities of non-inoculated anodes and anodes inoculated with sewage sludge. Analysis of the barcoded pre-grown multispecies biofilms reveal that 99% of the original biofilm was detached after 20daysof operation with municipal wastewater. This is in contrast to previous experiments where a pre-grown biofilm of exoelectrogens was operated in batch mode. To implement pre-grown biofilms in continuous systems it will thus be necessary to reveal a window of process parameters in which typical exoelectrogenic microorganisms including model organisms can be kept and/or enriched on anodes.

KEYWORDS:

Anode; Geobacter; Inoculation; Microbial community; Microbial fuel cell; Pregrown biofilm; Shewanella; Wastewater

PMID:
27639235
DOI:
10.1016/j.biortech.2016.09.004
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center