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Anal Chim Acta. 2016 Oct 5;939:84-92. doi: 10.1016/j.aca.2016.08.031. Epub 2016 Aug 30.

High-performance fluorescence-encoded magnetic microbeads as microfluidic protein chip supports for AFP detection.

Author information

1
School of Life Sciences, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University, Tianjin 300072, China.
2
Department of Laboratory Medicine, Tianjin Medical University General Hospital, Tianjin, 300052, China.
3
Bioscience (Tianjin) Diagnostic Technology CO., LTD, Tianjin, 300300, China.
4
Department of Biomedical Engineering, School of Electronics and Information Engineering, Tianjin Polytechnic University, Tianjin, 300387, China.
5
Department of Laboratory Medicine, Tianjin Medical University General Hospital, Tianjin, 300052, China. Electronic address: huzhidong27@163.com.
6
School of Life Sciences, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin University, Tianjin 300072, China. Electronic address: jinchang@tju.edu.cn.

Abstract

Fluorescence-encoded magnetic microbeads (FEMMs), with the fluorescence encoding ability of quantum dots (QDs) and magnetic enrichment and separation functions of Fe3O4 nanoparticles, have been widely used for multiple biomolecular detection as microfluidic protein chip supports. However, the preparation of FEMMs with long-term fluorescent encoding and immunodetection stability is still a challenge. In this work, we designed a novel high-temperature chemical swelling strategy. The QDs and Fe3O4 nanoparticles were effectively packaged into microbeads via the thermal motion of the polymer chains and the hydrophobic interaction between the nanoparticles and microbeads. The FEMMs obtained a highly uniform fluorescent property and long-term encoding and immunodetection stability and could be quickly magnetically separated and enriched. Then, the QD-encoded magnetic microbeads were applied to alpha fetoprotein (AFP) detection via sandwich immunoreaction. The properties of the encoded microspheres were characterized using a self-designed detecting apparatus, and the target molecular concentration in the sample was also quantified. The results suggested that the high-performance FEMMs have great potential in the field of biomolecular detection.

KEYWORDS:

AFP; FEMMs; Fluorescent encoding and immunodetection stability

PMID:
27639146
DOI:
10.1016/j.aca.2016.08.031
[Indexed for MEDLINE]

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